Research Article

The Stimulative Effect of Yangjing Capsule on Testosterone Synthesis through Nur77 Pathway in Leydig Cells

Figure 2

Effects of YC on Nur77 promoter activity in MLTC-1 cells. MLTC-1 cells were transfected with 200 ng Nur77 promoter-Luc. Cells were treated with 0, 0.01, 0.1, and 1 mg/mL YC and 0.1 U/mL hCG and assayed for luciferase activity after 24 h. Luciferase activity was normalized by 10 ng phRL-TK Renilla luciferase expression vector to determine the transfection efficiency. Data are a percentage of the control set to 100%. Values are mean ± SD from three independent experiments. Compared with the control, and .
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