Sulforaphane Reverses the Expression of Various Tumor Suppressor Genes by Targeting DNMT3B and HDAC1 in Human Cervical Cancer Cells

Figure 1

Effect of SFN and 5-Aza-dC on DNMT3B in human cervical cancer cells (HeLa). (a) 2.5 μM of SFN and 1.5 μM 5-Aza-dC treatments significantly inhibit the activity of DNMT in time-dependent manner, respectively. Values are means ± SD of three independent experiments. Symbol () indicates significant () difference of data between control and treated cells. (b) 2.5 μM SFN treated HeLa cells show significantly time-dependent reduction in the mRNA expression of DNMT3B in comparison to untreated cells. Panel A shows β-actin expression as an internal control, Panel B shows the expression of DNMT3B on treatment with 5-Aza-dC, and Panel C shows the expression of DNMT3B on treatment with SFN. Lane 1 shows the expression of DNMT3B gene in untreated HeLa cells; Lanes 2, 3, and 4 show the time-dependent alteration in the expression of DNMT3B after treatment for 24, 48, and 72 h, respectively; Lane 5 shows negative control for RT-PCR.

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