Sulforaphane Reverses the Expression of Various Tumor Suppressor Genes by Targeting DNMT3B and HDAC1 in Human Cervical Cancer Cells

Figure 6

Alterations of methylation status and mRNA expression levels of RARβ, CDH1, DAPK1, and GSTP1 genes after treatment with SFN. (a) mRNA expression levels before and after the treatment. Lane 1 shows the expression of these genes in untreated HeLa cells; Lanes 2, 3, and 4 show the time-dependent modulation in the expression of HDAC1 upon treatment for 24, 48, and 72 h, respectively; Lane 5 shows negative control for RT-PCR. β-actin was used as an internal control. (b) Methylation-specific bands (M) and unmethylation-specific bands (U). Lane 1 shows the methylation status of these genes in untreated HeLa cells; Lanes 2, 3, and 4 show the time-dependent modulation in the methylation status of RARβ, CDH1, DAPK1, and GSTP1 genes for 24, 48, and 72 h, respectively.

(a)

(b)