Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway

Figure 1

The effect of SCU on the protein level of PKG-I, VASP, and p-VASP protein in normal HBMECs. (a) Representative immunoblot and quantification of PKG-I, p-VASP, and VASP protein expression in normal HBMECs with SCU treatment. (b) The ratio of p-VASP and VASP compared with control in normal HBMECs with SCU treatment. Protein ratio of control was calculated by determining band integrated intensity as ratio of control. (c) The ratio of p-VASP compared with VASP in normal HBMECs with SCU treatment. (d) The ratio of PKG-I compared with control in normal HBMECs with SCU treatment. Protein ratio of control was calculated by determining band integrated intensity as ratio of control. Normal HBMECs, under normal culture condition: cells were incubated with SCU (0.1, 1, and 10 μM) for 24 h except control group. One-way ANOVA on Rank followed by SNK test, , , compared to control. Data are means ± SEM; independent experiments with independent culture.

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