Suppression of Propionibacterium acnes-Induced Dermatitis by a Traditional Japanese Medicine, Jumihaidokuto, Modifying Macrophage Functions

Figure 4

Immunofluorescent staining of macrophages 24 h after P. acnes injection. Pinnae sectioned at 4 μm were incubated with a PE-labeled anti-rat macrophage subset monoclonal antibody (clone: HIS36) and then mounted with antifade reagent with DAPI. Representative images merged with DAPI are shown for saline + DW (a), P. acnes + DW ((b) and (c)), P. acnes + 0.5 g/kg jumihaidokuto (JHT) ((d) and (e)), and P. acnes + 10 mg/kg prednisolone (PDN) ((f) and (g)). Panels (b), (d), and (f) are images of the inside of abscesses, and panels (c), (e), and (g) are the outside of abscess. Arrowheads indicate macrophages, which stained double positively with PE and DAPI. PE-positive and DAPI-negative cells found in the images are thought to be red blood cells and regarded as false positives for analysis of macrophages. Scale bars: 20 μm.