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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 453423, 7 pages
Research Article

Metabolomic Analysis of Clinical Plasma from Cerebral Infarction Patients Presenting with Blood Stasis

1Medical Research Division, Korean Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea
2Research Group of Nutrition and Metabolic Systems, Korean Food Research Institute, 62 Anyangpangyo-ro, 1201 Beon-gil, Bundang-gu, Seongnam, Gyeonggi-do 463-746, Republic of Korea
3Department of Food Biotechnology, University of Science & Technology, 62 Anyangpangyo-ro, 1201 Beon-gil, Bundang-gu, Seongnam, Gyeonggi-do 463-746, Republic of Korea

Received 29 October 2014; Revised 17 February 2015; Accepted 18 February 2015

Academic Editor: Hongcai Shang

Copyright © 2015 Min Ho Cha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Blood stasis (BS) is characterized as a disorder of blood circulation. In traditional Korean medicine (TKM), it is viewed as a cause factor of diseases such as multiple sclerosis and stroke. This study investigated differences in the plasma metabolites profiles of subjects displaying BS or non-BS patterns. Thirty-one patients with cerebral infarction diagnosed with BS and an equal number of sex- and age-matched non-BS patients were enrolled. Metabolic profiling was performed using UPLC-MS. The ratio of subjects with a rough pulse and purple coloration of the tongue was higher in patients presenting with BS pattern. Through metabolomics analysis, 82 metabolites that differed significantly between the BS and non-BS pattern were identified, and the two groups were significantly separated using an orthogonal partial least square-discriminant analysis model . Of these 82 metabolites, acetyl carnitine, leucine, kynurenine, phosphocholine, hexanoyl carnitine, and decanoyl carnitine were present in significantly higher levels in patients with a BS pattern than those with a non-BS pattern. Our results also demonstrated that seven plasma metabolites, including acyl-carnitines and kynurenine, were associated with a BS pattern, suggesting that variant plasma metabolic profiles may serve as a biomarker for diagnosis of BS in patients with cerebral infarction.