Pien Tze Huang Inhibits Hypoxia-Induced Angiogenesis via HIF-1α/VEGF-A Pathway in Colorectal Cancer

Figure 2

Effect of PZH on the migration in HUVECs. After treatment with the indicated concentrations of PZH for 24 h, the migration pattern of HUVECs was observed using phase contrast microscopy, wound-healing, and transwell migration assays. (a) A confluent monolayer of HUVEC was scratched in the middle with a pipette tip and the scratched area covered by cells that had migrated after exposure to normoxia or hypoxia for 12 h was measured; photographs are at 100x magnification. Images are representative of three independent experiments. (b) Transwell migration assays of HUVECs. Images are at 100x magnification. Images are representative of three independent experiments. (c) Migration distance after wound relative to the control at time of wound. ^#A significant difference compared with the normoxic control (). A significant difference compared with the hypoxic control (). (d) Measurement of the average number of migrating cells per field was assessed by counting 5 random fields. Statistics are expressed as the mean ± SD. ^#A significant difference compared with the normoxic control (). A significant difference compared with the hypoxic control ().

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