Pien Tze Huang Inhibits Hypoxia-Induced Angiogenesis via HIF-1α/VEGF-A Pathway in Colorectal Cancer

Figure 4

Effects of PZH on HIF-1, VEGF-A, and VEGFR2 expression in HUVECs. (a) mRNA expression levels of HIF-1 were analyzed by RT-PCR, and -tubulin was used as a loading control. (b) Protein expression levels of HIF-1α and VEGFR2 were analyzed by Western blot, and -actin was used as a loading control. (c) Secreted VEGF protein was analyzed by ELISA. ^#A significant difference compared with normoxic control (). A significant difference compared with hypoxic control ().

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