Effects of Chung-Pae Inhalation Therapy on a Mouse Model of Chronic Obstructive Pulmonary Disease
Effect of CP on the mRNA levels of cytokines in the lung of PPE- and LPS-induced COPD mice. Mice were exposed to PPE (on days 1, 7, and 14) and LPS (on days 4, 11, and 18) and administered 5 mg/kg or 20 mg/kg of CP 2 h after every LPS administration. The lungs of variously treated mice were harvested on day 21 for RT-PCR analysis. The intensity of each PCR band was measured by densitometric analysis (a), and relative expression of each gene was calculated over GAPDH. CP reduced the mRNA level of these cytokines (b–e). Data are presented as means ± SEM (). Letters (A–C) indicate different levels of significance (95% level; Duncan’s test).