Anti-Inflammatory and Cytostatic Activities of a Parthenolide-Like Sesquiterpene Lactone from Cota palaestina subsp. syriaca

Figure 6

Effect of K100 versus PTL on the proliferation rate, morphology (% of stellate versus spherical colonies), and size of colonies in 3D culture: (a) effect of K100 versus PTL on the proliferation rate of MDA-MB-231 cells in 3D culture conditions on day 8 of culture. Bar graph showing number of untreated, K100-treated, and PTL-treated MDA-MB-231 cells. (b) Effect of K100 versus PTL on the morphology of MDA-MB-231 cells in 3D culture conditions after three days. Phase contrast photomicrographs of MDA-MB-231 cells showing stellate colonies (A) of untreated cells and spherical colonies (B) obtained upon treatment with K100 or PTL. Histogram showing percent stellate versus spherical colonies of untreated and K100- or PTL-treated MDA-MB-231 cells at day 3. (c) Histogram showing colony sizes analysis, after grouping colonies in 3D cultures into small (less than 70 m), medium (between 70 and 100 m), and large (greater than 100 μm) clusters. Statistical significance is represented by () asterisk indicating significant difference at , () asterisk indicating significant difference at , and () asterisk indicating significant difference at .

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