Evidence-Based Complementary and Alternative Medicine

Research Article

Mechanistic Study of the Phytocompound, 2-β-D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches

Table 1

Differentially expressed proteins in GHTT-treated Jurkat T cells identified using 2DE/MS analysis.


ID	Protein name	Accession number	Theor. Mr (kDa)	Theor. pI	Sequence coverage (%)	Number of peptides matched	Avg. of fold difference^a	Functional categorization^b	Identification method

GDIR2	Rho GDP-dissociation inhibitor 2	P52566	22974	5.10	41	5	+4.0	Signal transduction	MS, MS/MS

GSTO1	Glutathione S-transferase omega 1	P78417	27548	6.23	44	12	+2.0	Detoxification	MS, MS/MS, ESI-MS/MS

HBB	Hemoglobin beta chain	P68871	15871	7.26	15	4	+3.4	Transport	MS/MS

PDIA3	Protein disulfide isomerase A3 precursor	P30101	56747	5.98	28	15	++	Metabolism	MS/MS

ADA	Adenosine deaminase	P00813	40555	5.54	35	14	+3.2	Metabolism; energy pathways	MS, MS/MS

SERA	D-3-phosphoglycerate dehydrogenase	O43175	56483	6.31	23	10	+2.3	Metabolism; energy pathways	MS, MS/MS

ECHM	Enoyl-CoA hydratase, mitochondrial precursor	P30084	31379	8.34	33	7	+2.9	Metabolism; energy pathways	MS, MS/MS

PARK7	DJ-1 protein (oncogene DJ1)	Q99497	19878	6.33	42	7	+1.6	Nucleoside, nucleotide, and nucleic acid metabolism	MS, MS/MS

IDHC	Isocitrate dehydrogenase [NADP] cytoplasmic	O75874	46630	6.53	28	9	+1.7	Metabolism; energy pathways	MS, MS/MS

LTOR3	Mitogen-activated protein kinase kinase 1 interacting protein 1	Q9UHA4	13614	6.73	85	8	+1.5	Signal transduction	MS, MS/MS

LMNB1	LMNB1 protein	AAH78178	38118	5.37	26	9	+5.1	Miscellaneous	MS, MS/MS

GSTP1	Glutathione S-transferase P	P09211	23210	5.44	40	6	+1.8	Metabolism; energy pathways	MS, MS/MS, ESI-MS/MS

VDAC2	Voltage-dependent anion-selective channel protein 2	P45880	38069	6.32	9	7	+1.5	Transport	MS, MS/MS

RM39	Mitochondrial 39S ribosomal protein L39	Q9NYK5	34204	6.47	26	7	—	Metabolism	MS, MS/MS

MCCB	Methylcrotonoyl-CoA carboxylase beta chain	Q9HCC0	61294	7.57	31	12	—	Metabolism; energy pathways	MS, MS/MS

TXNL1	Thioredoxin-like protein 1	O43396	32100	4.84	58	10	−4.4	Metabolism	MS, MS/MS

C3orf60	Chromosome 3 open reading fragment 60	AAH02873	20337	8.48	29	5	−2.1	Miscellaneous	MS, MS/MS

BID	BH3 interacting domain death agonist	P55957	21981	5.27	41	7	−3.8	Apoptosis	MS, MS/MS

PRDX3	Thioredoxin-dependent peroxide reductase, mitochondrial precursor	P30048	27675	7.67	14	3	−3.6	Metabolism; energy pathways	MS/MS

PPID	40 kDa peptidyl-prolyl cis-trans isomerase	Q08752	40607	6.76	36	12	−2.0	Metabolism; energy pathways	MS, MS/MS, ESI-MS/MS

GLRX3	Thioredoxin-like protein 2	O76003	37408	5.31	34	13	−2.7	Metabolism	MS, MS/MS, ESI-MS/MS

GLRX3	Thioredoxin-like protein 2	O76003	37408	5.31	31	10	−3.5	Metabolism	MS, MS/MS, ESI-MS/MS

NDUA5	NADH-ubiquinone oxidoreductase 13 kDa-B subunit	Q16718	13319	5.76	49	7	−2.1	Metabolism; energy pathways	MS/MS

^aData were obtained from the analysis of protein spots (GHTT versus control) using PDQuest software. Symbol — represents that the respective protein spot in the GHTT gel was not detected.
^bProteins were queried in Human Protein Reference Database (http://www.hprd.org/) for their functional categorization.