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Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 483980, 6 pages
http://dx.doi.org/10.1155/2015/483980
Research Article

Protective Effect of Artemisia asiatica Extract and Its Active Compound Eupatilin against Cisplatin-Induced Renal Damage

1College of Korean Medicine, Gachon University, Seongnam 461-701, Republic of Korea
2Department of Surgery, University of Ulsan College of Medicine, Gangneung Asan Hospital, Gangneung 210-711, Republic of Korea
3Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea
4Natural Products Research Center, Korea Institute of Science and Technology, Gangneung 210-340, Republic of Korea
5School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea
6Department of Pathology, University of Ulsan College of Medicine, Gangneung Asan Hospital, Gangneung 210-711, Republic of Korea

Received 13 April 2015; Accepted 14 June 2015

Academic Editor: Avni Sali

Copyright © 2015 Jun Yeon Park et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study investigated the renoprotective effect of an Artemisia asiatica extract and eupatilin in kidney epithelial (LLC-PK1) cells. Although cisplatin is effective against several cancers, its use is limited due to severe nephrotoxicity. Eupatilin is a flavonoid compound isolated from the Artemisia plant and possesses antioxidant as well as potent anticancer properties. In the LLC-PK1 cellular model, the decline in cell viability induced by oxidative stress, such as that induced by cisplatin, was significantly and dose-dependently inhibited by the A. asiatica extract and eupatilin. The increased protein expressions of phosphorylated JNK and p38 by cisplatin in cells were markedly reduced after A. asiatica extract or eupatilin cotreatment. The elevated expression of cleaved caspase-3 was significantly reduced by A. asiatica extract and eupatilin, and the elevated percentage of apoptotic cells after cisplatin treatment in LLC-PK1 cells was markedly decreased by cotreatment with A. asiatica extract or eupatilin. Taken together, these results suggest that A. asiatica extract and eupatilin could cure or prevent cisplatin-induced renal toxicity without any adverse effect; thus, it can be used in combination with cisplatin to prevent nephrotoxicity.