Protective Effect of Artemisia asiatica Extract and Its Active Compound Eupatilin against Cisplatin-Induced Renal Damage

Figure 2

Involvement of the MAPKs-caspase-3 signaling pathway in the protective effect of A. asiatica extract and eupatilin against cytotoxicity in cultured LLC-PK1 cells. Results of the Western blot show the levels of p-p38, p38, p-JNK, JNK, p-ERK, ERK, and cleaved caspase-3 in LLC-PK1 cells treated with A. asiatica extract and eupatilin and/or cisplatin at different concentrations for 24 h. Whole cell lysates (20 μg) were separated by SDS-PAGE, transferred onto PVDF transfer membranes, and probed with the indicated antibodies. Proteins were visualized using an ECL detection system.