Oldenlandia diffusa Promotes Antiproliferative and Apoptotic Effects in a Rat Hepatocellular Carcinoma with Liver Cirrhosis

Figure 1

The antitumor effect on hepatocellular carcinoma (HCC) cell lines treated with Oldenlandia diffusa (OD). The cytotoxic effect and antiproliferative activity were measured by CCK-8 cytotoxicity assay and immunoreactive cells such as Ki67 and caspase-3. The cytotoxicity effect on HCC cell lines treated with the indicated concentrations of OD (0–500 mg/mL) for 24 h (a). Also, we measured antiproliferative activity of OD (200 mg/mL) against HCC cells for 72 h (b). Ki67 and caspase-3 staining were performed after 24 h in OD (200 mg/mL) ((c), (d); scale bars, 100 μm). OD treatment in huh7 and hepG2 cells reduced ki67-positive cells and increased caspase-3 immunoreactivity. Columns, mean; bar, SE. Student’s -test, and .

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(b)

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