Oldenlandia diffusa Promotes Antiproliferative and Apoptotic Effects in a Rat Hepatocellular Carcinoma with Liver Cirrhosis

Figure 6

Liver morphology, liver/body ratio, and histopathology analysis 60 days after OD treatment. Liver/body weight ratio was measured after last OD treatment (200 mg/kg). Liver morphology was shown 60 days after OD treatment (200 mg/kg) and H&E staining was done. The large tumor observed in two sites (a). Except for tumor site, the rest of the liver tissue was similar to the normal liver morphology. OD (200 mg/kg) inhibits metastasis of tumor. Liver/body weight ratio in rats 60 days after OD treatment (b). The liver/body ratio after OD treatment (200 mg/kg) was not different between 28 days and 60 days (columns, mean; bar, SD). We show that tumor growth including the malignant nodules over 3 mm of a diameter, cirrhosis, and necrosis in tumor were significantly progressing in slides at 28 days in control group ((c)–(f)). 60 days after OD treatment, we observed the boundary between the large tumor and normal liver cells ((g)-(f), (k)–(m)). Also, no cirrhosis was shown and normal liver cells exist ((j), (n)). Scale bars ((a), (g), and (k)); 5 mm ((d), (h), and (l)); 500 μm ((e)-(f), (i)-(j), and (m)-(n)); 50 μm.

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