Terminalia chebula Fructus Inhibits Migration and Proliferation of Vascular Smooth Muscle Cells and Production of Inflammatory Mediators in RAW 264.7

Figure 3

Effect of TFW on VSMC migration. (a) Subconfluent VSMCs were scraped off with a pipette tip to induce wounds and then the cells were treated with TFW for 1 h followed by PDGF-BB treatment. Images of wounded area were captured immediately (time 0) and 6, 12, and 24 h after injury. (b) Finally wound closure was quantified as percentage of the initial wound area that had been recovered with VSMCs. Values are expressed as mean ± SD (). and versus control (PDGF-BB alone); original magnification 200x. (c) The cells were allowed to migrate in the presence of PDGF-BB and the indicated concentrations of TFW for 6 h. After staining with eosin and hematoxylin, the migrated cells were counted (d) in 5 high power fields (200x). Values are expressed as mean ± SD (). and versus control (PDGF-BB alone).

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