Terminalia chebula Fructus Inhibits Migration and Proliferation of Vascular Smooth Muscle Cells and Production of Inflammatory Mediators in RAW 264.7

Figure 4

Effect of TFW on MMP-2 and MMP-9 expression. VSMCs were pretreated with TFW for 1 h followed by stimulation with TNF-α for 24 h. (a) The proteolytic activity of MMP-2 and MMP-9 in conditioned medium and cell lysate was measured by gelatin zymography. The protein expression of MMP-9 was determined by Western blot. GAPDH was used for normalization. (b) Normalized quantitative data for MMP-9 protein expression. (c) Serum starved VSMCs were pretreated with indicated concentrations of TFW for 1 h followed by stimulation with PDGF-BB for additional 15 min. The cell lysates were assayed for protein expression of pFAK, FAK, RhoA, and Cdc42. (d) Normalized quantitative data for FAK activation. (e) Normalized quantitative data for Cdc42 expression. (f) Normalized quantitative data for RhoA expression. Values are expressed as mean ± SD; . and versus control (PDGF-BB alone).

(a)

(b)

(c)

(d)

(e)

(f)