Terminalia chebula Fructus Inhibits Migration and Proliferation of Vascular Smooth Muscle Cells and Production of Inflammatory Mediators in RAW 264.7

Figure 5

Effect of TFW on PDGF-BB induced VSMC proliferation. (a) Serum starved VSMCs were pretreated with indicated concentrations of TFW followed by stimulation with PDGF-BB (20 ng/mL) for 24 h. Cell proliferation was measured by MTT assay and values are expressed as mean ± SD; . and versus control (PDGF-BB alone). (b) Serum starved VSMCs were treated with indicated concentrations of TFW without stimulation for 24 h. Cell viability was measured by MTT assay. (c) Serum starved VSMCs were pretreated with indicated concentrations of TFW for 1 h followed by stimulation with PDGF-BB for additional 15 min. The cell lysates were assayed for protein expression of p-ERK1/2, ERK1/2, pJNK, and JNK. Values are expressed as mean ± SD; . versus normal (untreated).

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