Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects

Figure 5

CYP1A1 associated ethoxyresorufin-O-deethylase activity in the absence and presence of different concentrations of Ulva fasciata. (a) Reaction in a final volume of 200 μL was monitored for 10 min recording the fluorescence signal each 15 s. The reactions consisted of 1 pmol Supersome protein, 50 mM NADPH, and ethoxyresorufin at different concentrations. For the inhibition experiments, the extract was added at different concentrations to the reaction mixture. (b) Lineweaver-Burk plot analysis was performed to obtain the kinetic parameters. Each point in (a) represents the mean ± SD obtained from three independent experiments.

(a)

(b)