Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects

Figure 6

Confirmation of the CYP1A1 inhibitory properties of Ulva fasciata extract by (a) Dixon plot and (b) Y-intercept of the Linewaver-Burk plot versus inhibitor’s concentration. Each plot was obtained from independent reactions containing the desired concentration of ethoxyresorufin (0.31–5.00 μM), 1 pM Supersome protein, 50 mM NADPH, and different concentrations of the extract in a final volume of 200 μL. Each reaction was followed for 10 min, with the fluorescence signal being recorded every 15 s. Each point in (a) represents the mean ± SD obtained from three independent experiments. Slope of each plot in (a) was obtained and plotted versus the inverse of the concentration of ethoxyresorufin (b).

(a)

(b)