Free Radical Scavenging Activity: Antiproliferative and Proteomics Analyses of the Differential Expression of Apoptotic Proteins in MCF-7 Cells Treated with Acetone Leaf Extract of Diospyros lycioides (Ebenaceae)

Figure 10

2D-PAGE images of proteins extracted from MCF-7 cells (a) untreated and (b) 25 μg/mL of DL extract. Proteins were separated in the first dimension (1D) at pH 3–10 and on a 15% SDS-PAGE in the second dimension. Protein spots (circled) were further analysed by MALDI-TOF mass spectrometry. 2D-PAGE images of proteins extracted from MCF-7 cells (c) 65 μg/mL of DL extract and (d) positive control, curcumin. Proteins were separated in the first dimension (1D) at pH 3–10 and on a 15% SDS-PAGE in the second dimension. Protein spots (circled) were further analysed by MALDI-TOF mass spectrometry. The peptide sequences of the identified spots are presented in Spot 1, Spot 2, and Spot 3.

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