Proteomic Analysis of Anticancer TCMs Targeted at Mitochondria

Figure 3

Scheme of approaches for the application of proteomics in the mechanistic study of TCMs. (a) Overview of conventional quantitative proteomics on TCMs including SILAC, label-free, and iTRAQ. Among them, SILAC is suitable for cell models, while label-free and iTRAQ can be applied to samples from various sources. Samples can be analyzed by MS after trypsin digestion. After comparison, the differently expressed proteins are sorted out by bioinformatics and selected for further mechanism studies. (b) The procedure of modification proteomics on TCMs investigation, with phosphoproteomics as a widely used approach. Samples are digested into peptides, followed by enrichment and MS analysis. (c) Abridged general view of target fishing. Beads linked with certain components of TCMs are used to capture “preys” in whole cell lysates; the target proteins can then be identified by mass spectrometry.

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