Preclinical Evaluation on the Tumor Suppression Efficiency and Combination Drug Effects of Fermented Wheat Germ Extract in Human Ovarian Carcinoma Cells

Figure 2

Activation of cell death associated with the proteins, caspase-3, and caspase-7 as well as PARP, on FWGE-treated SKOV-3 and ES-2 cells. (a) SKOV-3 and ES-2 cells were treated with 200 μg/mL or 600 μg/mL of FWGE, respectively, for 72 h. The cleaved PARP was 89 kDa, the cleaved caspase-3 was 19 kDa, and the cleaved caspase-7 was 30 kDa, as indicated by the arrows; (b) the semiquantitation of GAPDH expression and the activation of caspase-3 and -7 in FWGE-treated SKOV-3 and ES-2 cells. Data were normalized with β-actin expression and were presented as the induction-fold compared with the control group (cells treated with the normal culture medium). ∗ indicates the statistical significance, as analyzed using the two-tailed Student’s -test ().

(a)

(b)