Type I Saikosaponins A and D Inhibit Osteoclastogenesis in Bone Marrow-Derived Macrophages and Osteolytic Activity of Metastatic Breast Cancer Cells

Figure 3

Effects of saikosaponins on RANKL-induced osteoclast formation in BMMs. (a) BMMs (5 × 10⁴ cells/well) were cultured in 10% FBS, α-MEM with M-CSF (30 ng/mL), RANKL (100 ng/mL), and each saikosaponin (5 μM). (b) BMMs were treated with saikosaponin A (SSA) or D (SSD) at the indicated concentrations. Control cells were treated with M-CSF alone. Five days later, RANKL-treated BMMs were stained for TRAP. TRAP-positive multinucleated (>3 nuclei) cells were photographed (original magnification, 100x) and counted. The number of osteoclasts is expressed as the mean ± SE. ^# versus control and ^* and ^** versus RANKL-treated BMMs.

(a)

(b)