Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 3

Research Article

Type I Saikosaponins A and D Inhibit Osteoclastogenesis in Bone Marrow-Derived Macrophages and Osteolytic Activity of Metastatic Breast Cancer Cells

Figure 3

Effects of saikosaponins on RANKL-induced osteoclast formation in BMMs. (a) BMMs (5 × 104 cells/well) were cultured in 10% FBS, α-MEM with M-CSF (30 ng/mL), RANKL (100 ng/mL), and each saikosaponin (5 μM). (b) BMMs were treated with saikosaponin A (SSA) or D (SSD) at the indicated concentrations. Control cells were treated with M-CSF alone. Five days later, RANKL-treated BMMs were stained for TRAP. TRAP-positive multinucleated (>3 nuclei) cells were photographed (original magnification, 100x) and counted. The number of osteoclasts is expressed as the mean ± SE. # versus control and * and ** versus RANKL-treated BMMs.

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