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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 584018, 10 pages
http://dx.doi.org/10.1155/2015/584018
Research Article

Antioxidant Formulae, Shengmai San, and LingGuiZhuGanTang, Prevent MPTP Induced Brain Dysfunction and Oxidative Damage in Mice

1J.K.K. Nattraja College of Pharmacy, Komarapalayam, Tamil Nadu 638 183, India
2Niigata University of Pharmacy & Applied Life Sciences, Niigata 956 8603, Japan
3Department of Cardiovascular Sciences, Houston Methodist Research Institute, Houston, TX 77030, USA
4Changchun University of Chinese Medicine, Bosuo Road No. 1035, Jingyue Economic Development District, Changchun 130117, China

Received 11 June 2015; Revised 12 August 2015; Accepted 11 October 2015

Academic Editor: Pierre Champy

Copyright © 2015 Vijayasree Vayalanellore Giridharan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study was designed to evaluate the preventive effect of antioxidative traditional oriental medicine formulae, Shengmai San (SMS) and LingGuiZhuGanTang (LGZGT), against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (i.p 30 mg·kg−1 for 5 consecutive days) induced neurotoxicity. In in vitro antioxidant assays measured with Trolox and butyl hydroxyl toluene as reference antioxidant revealed that SMS has higher scavenging potential against hydroxyl radical than superoxide anion radical, but LGZGT was the reverse. The neuroprotective effect of SMS and LGZGT against MPTP was evaluated in mice by behavioral, biochemical, and immunohistochemical studies. In the behavioral study, both SMS and LGZGT significantly reversed the locomotive impairment induced by MPTP. Simultaneously, both formulae significantly prevented the MPTP induced dopaminergic neuron loss assessed by tyrosine hydroxylase in the midbrain. Both SMS and LGZGT significantly attenuated the elevated lipid peroxidation and protein carbonyls levels by MPTP. The DNA damage induced by MPTP was also prevented by both formulae. Although a little difference in the protective functions was observed between the two formulae, such as in DNA damage and behavioral studies, the results indicate that both SMS and LGZGT with antioxidant property act as a good candidate applicable for the antioxidant based complementary therapies of neurodegenerative diseases.