Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes

Figure 3

Effects of hinokitiol on positive regulators of the cell cycle. Cells were treated with hinokitiol (1–5 μM) in the absence or presence of ConA (10 μg/mL) for 24 h. The specific antibodies were used to detect (a) cyclin D3, (b) Cdk4, and (c) E2F1. Data (a–c) are presented as the mean ± SEM (; and compared with solvent control (DMSO); ^#, ^##, and ^### compared with the ConA-treated group).

(a)

(b)

(c)