The Effect of Hydrocotyle sibthorpioides Lam. Extracts on In Vitro Dengue Replication

Table 1

Morphological changes in DENV-2 infected cells.

Cell line	Solvent	Concentration (µg/mL)	Dilution of extract	Degree of CPE manifestation
				Pretreatment		Posttreatment
				200 pfu	2000 pfu	200 pfu	2000 pfu
Vero	Water	20	1/64	+++	+++	+++	+++
		156	1/8	+++	+++	+++	+++
		1250	1/2	+++	+++	+++	+++
	Methanol	39	1/16	+++	+++	+++	+++
		156	1/4	+++	+++	++	++
		625	1/2	+++	+++	++	++
C6/36	Water	10	1/64	+++	+++	++++	++++
		78	1/8	+++	+++	++++	++++
		625	1/2	+++	+++	++++	++++
	Methanol	10	1/16	+++	+++	++++	++++
		39	1/4	+++	+++	++++	++++
		156	1/2	+++	+++	++++	++++

“++++” 100% virus growth-like CPE, “+++” 75% virus growth-like CPE, “++” 50% virus growth-like CPE, “+” <50% virus growth-like CPE, “−” no CPE.