Interferon-γ-Mediated Natural Killer Cell Activation by an Aqueous Panax ginseng Extract

Figure 1

Concentration of Rb1 and Rg1 in aqueous extract, 50% ethanol extract, and 95% ethanol extract. Standard sample was diluted to 0.001%. Each sample was diluted to 0.2%. High-performance liquid chromatography (HPLC) was performed under the following conditions according to the Ginseng section of the revised 16th edition of the Japanese Pharmacopoeia: injected volume, 20 μL each; detector, UV absorption spectrometer (203 nm); column, stainless, 150 × 4.6 mm; octadecyl-silica (ODS), 5 μm. Mobile phase, column temperature, and flow rate for ginsenosides Rb1 and Rg1 were water/acetonitrile (4 : 1) and water/acetonitrile (7 : 3), 30 and 40 degrees Celsius, and retention time of about 25 and 20 minutes, respectively.