(a)
(b)
(c)
Figure 4: Hepatic expression of cell cycle progression associated with silymarin (Sm) in liver regeneration by western blot and RT-PCR analysis. (a) Silymarin cannot hold cell cycle life in rat liver regeneration after partial hepatectomy in 72 hrs. Equal amounts of proteins lysate were separated by 12.5% SDS-PAGE by western blotting with antibodies to cyclin A. α-Tubulin was used as a load control for western blotting. Quantification of densitometry analysis of protein expression levels. All data are presented as means SEM, , as compared with the corresponding sham group. , as compared with the corresponding PHx. , as compared with 6 hrs sham group. as compared with 24 hrs sham group. , as compared with 6 hrs PHx group. , as compared with 24 hrs PHx group. (b) RT-PCR cyclin A mRNA expression analysis showed decrease after silymarin treatment after PHx at 24 hrs and 72 hrs. GAPDH was used as a load control for RT-PCR. Quantification of densitometry analysis of mRNA levels. All data are presented as means SEM, , as compared with the corresponding sham group. , as compared with the corresponding PHx. as compared with 6 hrs sham group. as compared with 6 hrs PHx group. as compared with 24 hrs PHx group.