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Figure 5: The effect of silymarin (Sm) on hepatocyte proliferation after PHx. (a) Silymarin (Sm) induced HGF protein and mRNA expression at 6 hrs PHx using western blot and RT-PCR analysis. Densitometry analysis quantification of protein and mRNA expression levels; all data are presented as mean ± SEM, as compared with the corresponding sham group. , as compared with the corresponding PHx. α-Tubulin was used as a loading control for western blotting. GAPDH was used as a load control for RT-PCR. (b) Silymarin (Sm) induced HGF protein and mRNA expression at 72 hrs PHx using western blot and RT-PCR analysis. Quantification of densitometry analysis of protein and mRNA expression levels; all data are presented as mean ± SEM, , as compared with the corresponding sham group. as compared with the corresponding PHx. α-Tubulin was used as a load control for western blotting. GAPDH was used as a loading control for RT-PCR. (c) Silymarin (Sm) induced TGFα protein expression from 6 to 72 h PHx by western blot.