(a)
(b)
Figure 6: TGFβ1 contributed in some way to the events leading hepatocytes from the G0 phase to the G1 phase of the cell cycle in liver regeneration after partial hepatectomy. (a) Equal amounts of protein lysates were separated by 12% SDS-PAGE by western blotting with antibodies to TGFβ1. Protein expression levels were increased in treatment silymarin (Sm) after 6 hrs, 24 hrs, and 72 hrs PHx. Quantification of densitometry analysis of protein expression levels. All data are presented as means SEM, , , as compared with the corresponding sham group. as compared with the corresponding PHx. , as compared with 6 hrs PHx group. as compared with 24 hrs PHx group. α-Tubulin was used as a load control for western blotting. (b) RT-PCR analysis of TGFβ1 mRNA expression during liver regeneration. Quantification of densitometry analysis of mRNA expression levels. All data are presented as means SEM, , as compared with the corresponding sham group. as compared with the corresponding PHx. , as compared with 6 hrs sham group. as compared with 24 hrs sham group. , as compared with 6 hrs PHx group. as compared with 24 hrs PHx group. GAPDH was used as a loading control for RT-PCR.