- About this Journal ·
- Abstracting and Indexing ·
- Aims and Scope ·
- Annual Issues ·
- Article Processing Charges ·
- Articles in Press ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 615384, 9 pages
Bacopa monnieri as an Antioxidant Therapy to Reduce Oxidative Stress in the Aging Brain
1Centre for Human Psychopharmacology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia
2Department of Neurology, Boston University School of Medicine and Framingham Heart Study, Boston, MA 02118, USA
Received 19 January 2015; Revised 26 March 2015; Accepted 10 April 2015
Academic Editor: Abid Hamid
Copyright © 2015 Tamara Simpson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The detrimental effect of neuronal cell death due to oxidative stress and mitochondrial dysfunction has been implicated in age-related cognitive decline and neurodegenerative disorders such as Alzheimer’s disease. The Indian herb Bacopa monnieri is a dietary antioxidant, with animal and in vitro studies indicating several modes of action that may protect the brain against oxidative damage. In parallel, several studies using the CDRI08 extract have shown that extracts of Bacopa monnieri improve cognitive function in humans. The biological mechanisms of this cognitive enhancement are unknown. In this review we discuss the animal studies and in vivo evidence for Bacopa monnieri as a potential therapeutic antioxidant to reduce oxidative stress and improve cognitive function. We suggest that future studies incorporate neuroimaging particularly magnetic resonance spectroscopy into their randomized controlled trials to better understand whether changes in antioxidant status in vivo cause improvements in cognitive function.
The world’s population is aging rapidly . One consequence of an aging population is an increased prevalence of chronic, age-related illnesses and disorders involving oxidative stress and low level chronic inflammation . Increasing age is a major risk factor for dementia, including Alzheimer’s disease (AD), and other prevalent neurodegenerative disorders . The causes of brain aging and dementia are complex and incompletely understood.
Oxidative stress is one mechanism that detrimentally contributes to the aging process and is inextricably linked to neurodegenerative disorders . Interventions that manipulate the oxidative stress mechanisms may decrease oxidative damage, slow the rate of aging, and lessen the risk of neurodegenerative disorders, increasing the lifespan of older adults. Research has begun to focus on developing effective health and lifestyle interventions so that older adults are able to remain both physically and cognitively healthy into older age, reducing the social and economic burden associated with an aging population .
The Indian herb, Bacopa monnieri (EBm) may serve as a dietary antioxidant, with several modes of action to protect the brain against oxidative damage and age-related cognitive decline. Several studies using the standardized CDRI08 extract have shown that EBm improves cognitive function particularly in the elderly [6–8]. Animal and in vitro studies using the standardized extract CDRI08 have revealed promising results to elucidate EBm’s antioxidant properties (e.g., [9–12]). The aim of this review is to examine the evidence for EBm as a potential therapeutic antioxidant to reduce oxidative stress in the aging brain and as a mechanism by which it may improve cognition. We also discuss magnetic resonance spectroscopy (MRS) as a technique to elucidate the antioxidant mechanisms of action of EBm in human research in vivo.
2. The Aging Brain
Deterioration in memory performance is a signature of advancing age. Almost 50% of adults aged 64 years and over tend to report difficulties with their memory . In addition to memory, executive function, processing speed, attention, and spatial ability have also been shown to deteriorate with age [14–19]. While most cognitive functions decline with age, cognitive aging does not occur uniformly at the same rate and to the same extent for all people . There may be several reasons for this including differences in lifestyle factors, particularly dietary intake.
Aging is a predominant risk factor for dementia, including AD . An imbalance between the production and clearance of abnormal proteins called -amyloid , the formation of neurofibrillary tangles, and neuroinflammation are hallmarks of advanced brain aging and dementia . Neuroimaging studies have reliably identified that with increasing age, ventricular enlargement, white matter hyperintensities, reduction in gross brain volume, reductions in frontal and temporoparietal volume, and higher levels of cortical atrophy occur in the brain . The shrinkage of cortical volume is believed to impact cognitive functioning negatively, reducing a person’s cognitive ability . Functional magnetic resonance imaging studies have revealed that age-related memory changes may be due to altered activation of the prefrontal cortex (PFC). For example, compared to their younger counterparts, older adults recruit a broader area of the PFC due to bilateral activation of this region . Cabeza  conceptualized this phenomenon as a reduction in hemispheric asymmetry, a compensatory response to a loss in neural efficiency. Other researchers explain older adult’s broad brain activation during working memory tasks with the compensation-related utilization of neural circuits hypothesis (CRUNCH) . This hypothesis suggests that the compensatory response of the brain’s bilateral over activation occurs due to older adults recruiting maximal neuronal resources earlier than younger adults when completing the same tasks, thereby leaving no neuronal resources left for a higher load of difficulty, resulting in poorer performance. Since these two theories, researchers believe the compensatory response is in fact a protective scaffolding effect to support or prop up underlying adverse factors associated with brain ageing like brain shrinkage, reduction in dopamine receptors, neural inefficiency, noise, or both (scaffolding theory of aging and cognition (STAC)) . To enable researchers to predict cognitive status and change over time, a revised STAC model (STAC-r) incorporates aging and life experience factors like exercise and cognitive training that influence structure and function of the aging brain which in turn may enhance or deplete neural resources .
More recently, neuroimaging studies have investigated the correlation between brain neurometabolite levels, as an indication of underlying molecular or cellular changes that may be related to aging. The technique of MRS is a noninvasive method of obtaining biochemical information about body tissue . MRS has been used to study age-related degenerative diseases like cognitive impairment and AD [31, 32] and neuropsychiatric disorders like depression  and schizophrenia . MRS can be utilized for early detection of disease and for monitoring medical therapies or treatments . Changes in metabolites are purported to reflect changes in different brain indices such as neuronal viability/function (N-acetyl-aspartate; NAA), cellular turnover (Choline; Cho), metabolic activity (glutamate, glutamine; GLX), inflammation in the brain (myo-inositol; Myo), and oxidative stress (glutathione; GSH) .
MRS studies have investigated the correlation between changing brain neurometabolite levels and cognitive performance in healthy aging populations [37, 38]. A study by Ross and colleagues  identified significant correlations between the integrity of frontal white matter NAA metabolite and cognitive function represented by processing speed, visual memory, and attention tasks with a healthy elderly cohort. A large study conducted by Ferguson and colleagues  investigated the relationships between NAA, Cho, and Cr and cognitive function in a group of healthy elderly men. Positive correlations were found between NAA/Cr and Cho/Cr ratios with test measures of logical and verbal memory. The authors postulated that high levels of Cr are the best predictor of poor cognitive performance. An increase in the Cr signal has been reliably identified in healthy elderly brains compared with their younger counterparts [41–43]. These studies support the premise that MRS is a valid technique to measure subclinical changes in cognition across the lifespan.
Alternatively, researchers investigating MRS metabolite markers in clinical cohorts with Alzheimer’s disease (AD) have reliably found NAA to be lower and Myo to be higher when compared to cognitively healthy older adults (e.g. [31, 41]). However, inconsistent Cho levels have been identified with some studies reporting an increase in Cho of people with AD  while others have not . Collectively, these physiological, pathophysiological, and structural changes that occur with increasing age highlight the complex nature of the aging brain.
Understanding the mechanisms and role of oxidative stress in the aging process is currently considered to be important to elucidate the key to longevity. An emerging theory in the literature postulates that the balance between oxidation/reduction reactions (redox state) within cells is important for healthy aging . If there is a disruption to the mechanisms of redox state (impaired signalling and regulation), then age-associated functional losses will occur . Oxidative stress, antioxidants, and the aging brain will be discussed below.
3. Oxidative Stress, Antioxidants, and the Aging Brain
3.1. Oxidative Stress Mechanisms
Although oxygen is needed for survival, the brain is sensitive to oxygen metabolic activity that produces ROS . Approximately 95%–98% of ROS such as hydrogen peroxide (H2O2), hydroxyl free radical (OH), superoxide anion (), and peroxynitrite () are formed in mitochondria as by-products of cellular respiration . Studies of mitochondria isolated from the brain indicate that 2–5% of the total oxygen consumed produces ROS . An imbalance between prooxidant and antioxidant reactions occurs when the equilibrium between the beneficial and harmful effects (redox homeostasis) is interrupted (; refer to Figure 1). This imbalance produces a steady accumulation of oxidative damage in macromolecules that increase with aging, causing a progressive loss in cellular function and efficiency of processes . Free radicals in the brain are responsible for significant harmful effects to cellular function and damage to DNA, proteins, membrane lipids, and components of mitochondria [47, 52]. The brain is particularly sensitive to free radical damage due to its high metabolic rate, concentration of unsaturated fatty acids, cytotoxic actions of glutamate and reduced antioxidant systems with a lower activity of glutathione peroxidase (GPx) and catalase (CAT) compared to other organs [9, 53].
Aging decreases the ability of the brain to scavenge free radicals, thus decreasing available antioxidants, particularly the most abundant endogenous antioxidant GSH [47, 50]. There is a delicate balance between the positive and negative effects of free radicals. In a normal physiological state, fluctuation in ROS production is balanced with ROS scavenging capacity . Oxidative stress occurs when ROS production exceeds that of ROS scavenging capacity. Oxidative stress is a significant feature of aging, most likely due to a combination of reduced ROS scavenging capacity, impaired redox state, and increased ROS production . This imbalance in the cellular redox mechanisms may contribute to the slow onset and progressive nature of neurodegenerative diseases, as well as age-related cognitive decline [46, 56]. Severe, extensive, or more prolonged oxidative damage is highly toxic and these toxic effects contribute significantly to the aging process [57, 58].
3.2. Antioxidant Mechanisms
The human body has an innate defence mechanism consisting of endogenous antioxidants to negate the detrimental effects of oxidants . Antioxidants have the ability to reduce oxidative stress in the body by scavenging ROS to either inhibit or repair damage. Antioxidant enzymes superoxide dismutase (SOD), CAT, GPx, and glutathione reductase (GR) present the first line of defence against free radical damage under conditions of oxidative stress [60, 61]. Nonenzymatic antioxidants, glutathione (GSH), vitamin C (ascorbic acid), and vitamin E (-tocopherol) are all phenolic compounds that offer protection by altering oxidants to either nonradical end products or transporting radicals to areas where their effects will be less damaging . Vitamins A, C, and E, selenium, and coenzyme Q10 effect important antioxidant actions to protect neural tissue from “attack” by free radicals . Eating a varied diet can provide a mixture of oxidants and antioxidants. Fruits and vegetables rich in vitamins A, C, and E provide a healthy defence against the formation of free radicals. Such fruits and vegetables also increase the number of cell receptors available for antioxidant enzyme action . Dietary polyphenols with antioxidant properties have protective effects against many degenerative diseases including diabetes, cancer, and cardiovascular diseases aiding in the prevention of oxidative stress [63, 64].
The GSH redox cycle lowers H2O2 levels, thus lowering the formation of damaging hydroxyl radicals (; refer to Figure 2). GSH is a tripeptide (L--glutamyl-L-cysteinylglycine) found everywhere within the cells of the body. It is involved in many physiological functions. GSH is responsible for detoxifying ROS into nontoxic substances (water and oxygen)  and is critical for the maintenance of normal function and neuronal survival . It is involved in the synthesis of proteins and DNA, enzyme activity, metabolism, transport, and cell protection . GSH is oxidised to glutathione disulphide (GSSG) resulting in intracellular redox imbalance which is reflected in a decreased GSH to GSSG ratio, often referred to as oxidative stress . GSH levels in tissue decrease with age . Impaired GSH metabolism has been implicated in the pathogenesis of clinical mental disorders like schizophrenia and bipolar disorder  as well as neurodegenerative disorders including AD  and Parkinson’s disease . It is not surprising then that oxidative stress generated by ROS is consistently linked to these conditions.
A study conducted by Berger and colleagues  used MRS to investigate in vivo GSH levels before and after the administration of an omega-3 fatty acid (ethyl eicosapentaenoic acid; E-EPA) within patients who had experienced their first episode of psychosis. Supplementation with E-EPA was reported to increase GSH concentration by 38% in the temporal lobe of these patients. More importantly, the increase in GSH correlated with improvement in negative symptoms. This promising result provides support for further research to be conducted to elucidate the effect of other supplements on cerebral GSH levels in normal and clinical populations.
GSH levels, quantified using MRS, have been used to investigate oxidative stress in different brain regions (frontal cortex, parietal cortex, hippocampus, and cerebellum) of healthy adults and in the bilateral frontal cortices of patients with mild cognitive impairment and AD . In healthy females compared to healthy males, mean GSH levels were higher (left frontal cortex, ; right posterior cortex, ) and that GSH distribution was different between the hemispheres for females and males. GSH levels were significantly depleted in the right frontal cortex of female AD patients compared to healthy female participants (), whereas, for males, the left frontal cortex was significantly depleted () when comparing healthy males to AD patients. GSH was lower in mild cognitive impaired patients compared to healthy participants, but the difference was not statistically significant. GSH is therefore an important biomarker of redox state which can be monitored to investigate disease progression or normal age-related changes .
Despite the compelling research linking brain metabolite alterations to changes in cognitive function with age and changes in GSH levels in clinical populations, to date, no studies have incorporated the use of MRS as a technique to measure metabolite changes in response to EBm. Clinical trials have not investigated the antioxidant defence system, particularly targeting the ubiquitous antioxidant, GSH, in response to EBm using MRS. Finally, clinical trials have not examined the cognitive correlates of MRS after the chronic administration of EBm.
Altering the inefficiency of the antioxidant system by boosting the redox potential with thiols, particularly the ubiquitous antioxidant, GSH, may be a way to reduce age-associated decline in functional abilities. As discussed below, the administration of EBm may improve the antioxidant redox state, thus leading to improved functional outcomes such as enhanced cognitive performance.
4. What Is Bacopa monnieri?
Bacopa monnieri (Linn), commonly referred to as “Brahmi,” from the plant family Scrophulariaceae is a creeping herb found in India and neighbouring tropical countries that grows in wet marshland up to 1500 m in altitude . It has been traditionally used in Ayurvedic medicine to treat conditions such as fever, inflammation, pain, asthma, epilepsy, and memory decline . It has been used in a standardized form in clinical research since 1996 . Steroidal saponins and Bacosides A and B are the active chemical constituents responsible for improving both learning and memory [75, 76]. Other constituents include bacopasaponins D, E and F as well as alkaloids, flavonoids, and phytosterols [77, 78]. Some of the chemical constituents of EBm are lipophilic [79, 80]. This means that they can combine with or dissolve in lipids giving them the ability to cross the blood-brain barrier. Bacosides are believed to repair damaged neurons by enhancing kinase activity and neuronal synthesis linked with the restoration of synaptic activity, culminating in the improvement of nerve impulse transmission . Antidepressant and anxiolytic effects have been reported in animal studies [82, 83] although conflicting findings have been reported in human trials [6, 7, 84, 85]. However it is the memory enhancing effects of EBm that have generated the most attention . Various mechanisms may be involved in the neuroprotective and memory enhancing effects of EBm, such as the binding and detoxification of metal ions , free radical scavenging , or increasing antioxidant activity . Animal models have shown that EBm can exert vasorelaxant , adaptogenic , anti-inflammatory , metal ion chelating , and cholinergic modulatory effects . Neuroprotective effects have been identified in animal models of epilepsy  and amnesia  as well as reducing ischemia-induced memory deficits in rats . EBm also appears to inhibit numerous -amyloid oxidative stress pathways involved in AD pathology  and antioxidant properties related to GSH redox state . The role of oxidative stress and alterations in the antioxidant GSH redox state in response to EBm will be expanded upon below.
5. Antioxidant/Oxidative Stress Mechanisms of Bacopa
The antioxidant properties of EBm are widely recognised and have been discussed in various reviews [10, 98, 99]. Several histological (in vitro) and animal studies have established that EBm bacosides or extract improve the system’s defences against oxidative stress by decreasing the formation of free radical accumulation in the brain. In an early study investigating the antioxidant activity of EBm, lipid peroxidation in the prefrontal cortex, striatum, and hippocampus of rats was inhibited. Bhattacharya and colleagues  found a dose related increase in enzyme activity responsible for scavenging reactive oxygen species, namely, SOD, CAT, and GPx in these brain regions of rats after 14 and 21 days of chronic administration of EBm. Interestingly, the same study compared the antioxidant effects of the drug deprenyl, which also improved antioxidant enzyme activity, but only in the prefrontal cortex and striatum of the rats and not the hippocampus. They suggested that this increase in the free radical scavenging activity of bacosides may be responsible for facilitating the cognitive action of EBm. Similarly, in a different study, the modulation of antioxidant activity in diabetic rats was again through a significant increase in SOD, CAT, GPx, and GSH levels showing a significant reversal of redox imbalance and peroxidative damage to enhance the defence system against ROS . Other studies also support a free radical scavenging mechanism in response to EBm  by reducing the formation of free radicals [92, 100]. In addition, a more recent study found that a EBm ethanolic extract was able to adjust the level of endogenous oxidative markers in various brain regions of prepubertal mice .
Furthermore, an in vitro study by Russo and colleagues  investigated H2O2 induced cytotoxicity and DNA damage in human nonimmortalized fibroblast cells in response to an ethanol extract of EBm. They also investigated the free radical scavenging capacity and the effect on DNA cleavage induced by H2O2. EBm was able to inhibit superoxide anion formation in a dose dependent manner, indicative of free radical scavenging ability and a protective effect was observed against H2O2 cytotoxicity and DNA damage. A more recent in vivo and in vitro study conducted by Shinomol and colleagues  used 3-nitropropionic acid (NPA), a fungal toxin that causes neurotoxicity in both animals and humans, in comparison with the effects of an ethanolic extract of EBm in the mitochondria of the striatum of rats and dopaminergic (N27) cells. As predicted, the NPA caused oxidative stress in the mitochondria of the striatum, while pretreatment with EBm prevented NPA oxidative reactions and reduction of reduced GSH and thiol levels.
In experimental models of ischemia, diabetes and aluminium and cigarette induced toxicity, pretreatment with EBm (40 mg/kg/day to 250 mg/kg/day) and Bacoside A (10 mg/kg/day) has been identified to prevent lipid peroxidation and play a role in antioxidant activities by modulating the effects of enzymes (Hsp 70, cytochrome P450, and SOD in the rat brain) known to be involved in the production and scavenging of ROS, resulting in antistress activity in rats [103–105]. Again, these studies support the premise that EBm has the ability to restore antioxidant defence mechanisms and protect against the adverse effects of peroxidative damage. EBm has also been shown to either exert antioxidant effects through metal chelation at the initiation level of the free radical chain reaction by chelating ferrous ions, or be attributed to the detoxification of free radicals at the propagation level [87, 92]. In another study in rats, the effect of EBm detoxified ROS in astrocytes .
Cumulatively, animal and in vitro studies provide support for antioxidant mechanisms of EBm. Animal and in vitro studies have identified that GSH is particularly useful to examine antioxidant capacity and changes in oxidative stress. Taking into account the findings of the studies described above, EBm may increase the cellular inefficiency of the antioxidant system by boosting the redox potential with GSH (e.g., [9, 11]). In turn, the administration of EBm as a therapeutic intervention may be a way to reduce age-associated decline in functional abilities. The therapeutic properties of plants like EBm have generated much scientific investigation due to their compelling antioxidant properties, little to no side effects, and economic sustainability .
6. Clinical and Research Implications
Oxidative stress plays a role in aging and neurodegenerative disorders. Based on the animal in vitro and in vivo studies discussed in this review, EBm can be utilized as a therapeutic strategy against oxidative damage and cognitive decline in the elderly. Supplementation with EBm is likely to support the antioxidant defence pathways altering the redox status, which are vital components for normal functioning, while improving cognitive ability. Given that with age it is believed that the antioxidant system is compromised and GSH levels are reduced, EBm has the potential as a therapeutic antioxidant to reduce oxidative stress and improve cognitive performance.
7. Future Directions
While the central role of oxidative stress in age-related cognitive decline and neurodegenerative diseases has driven studies to examine the potential antioxidant benefits of EBm, studies have not incorporated in vivo brain imaging techniques to systematically study brain aging and central oxidative stress. MRS may be a useful technique to understand the antioxidant mechanisms, particularly studying GSH ROS detoxification, in vivo, as a result of EBm supplementation. Applying neuroimaging research techniques is important to be able to understand the in vivo effects underpinning the cognitive changes due to EBm. Future studies should consider the application of brain imaging modalities, particularly MRS, to be able to extend results beyond the explanation of mood, general health, and cognitive behavioural outcomes in response to dietary supplementation in human randomized clinical trials.
Further exploration into the complex mechanisms of action of EBm in nutritional aging studies may reveal promising insights into antioxidant metabolic changes, supporting dietary nutritional supplementation for therapeutic means. This review has described how EBm has the potential as a therapeutic antioxidant to reduce oxidative stress, a mechanism that may be responsible for improving cognitive performance and offer neuroprotection. Employing the neuroimaging technique of MRS to investigate GSH antioxidant levels may be useful to elucidate the mechanisms of action underlying the cognitive enhancing effects of EBm. Such research may also assist in our understanding of how to improve cognition in the elderly.
Conflict of Interests
The authors declare that there is no conflict of interests in this paper.
The authors would like to thank Professor Kevin Croft for his comments on the final draft in preparation for this paper. They would also like to thank Dr. Doug Mitchell for funding contributing to neuroimaging research on Bacopa monnieri (CDRI08). The authors conduct studies on Bacopa monnieri (CDRI08) that are funded by SOHO Flordis International (SFI). Tamara Simpson is funded by an Australian Postgraduate Research Award to conduct this research. Professor Stough’s work on Bacopa monnieri (CDR108) is funded by a large grant from the Australian Research Council (ARC) DP1093825.
- United Nations, World Population Ageing 2013, P.D. Department of Economic & Social Affairs, New York, NY, USA, 2013.
- N. S. Jenny, “Inflammation in aging: cause, effect, or both?” Discovery Medicine, vol. 13, no. 73, pp. 451–460, 2012.
- B. A. Yankner, T. Lu, and P. Loerch, “The aging brain,” Annual Review of Pathology: Mechanisms of Disease, vol. 3, pp. 41–66, 2008.
- N. Khansari, Y. Shakiba, and M. Mahmoudi, “Chronic inflammation and oxidative stress as a major cause of age-related diseases and cancer,” Recent Patents on Inflammation and Allergy Drug Discovery, vol. 3, no. 1, pp. 73–80, 2009.
- D. J. Lowsky, S. J. Olshansky, J. Bhattacharya, and D. P. Goldman, “Heterogeneity in healthy aging,” The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, vol. 69, no. 6, pp. 640–649, 2014.
- C. Stough, J. Lloyd, J. Clarke et al., “The chronic effects of an extract of Bacopa monniera (Brahmi) on cognitive function in healthy human subjects,” Psychopharmacology, vol. 156, no. 4, pp. 481–484, 2001.
- C. Stough, L. A. Downey, J. Lloyd et al., “Examining the nootropic effects of a special extract of Bacopa monniera on human cognitive functioning: 90 day double-blind placebo-controlled randomized trial,” Phytotherapy Research, vol. 22, no. 12, pp. 1629–1634, 2008.
- P. J. Nathan, S. Tanner, J. Lloyd et al., “Effects of a combined extract of Ginkgo biloba and Bacopa monniera on cognitive function in healthy humans,” Human Psychopharmacology, vol. 19, no. 2, pp. 91–96, 2004.
- S. K. Bhattacharya, A. Bhattacharya, A. Kumar, and S. Ghosal, “Antioxidant activity of Bacopa monniera in rat frontal cortex, striatum and hippocampus,” Phytotherapy Research, vol. 14, no. 3, pp. 174–179, 2000.
- A. Russo and F. Borrelli, “Bacopa monniera, a reputed nootropic plant: an overview,” Phytomedicine, vol. 12, no. 4, pp. 305–317, 2005.
- R. Kapoor, S. Srivastava, and P. Kakkar, “Bacopa monnieri modulates antioxidant responses in brain and kidney of diabetic rats,” Environmental Toxicology and Pharmacology, vol. 27, no. 1, pp. 62–69, 2009.
- H. K. Singh, “Brain enhancing ingredients from āyurvedic medicine: quintessential example of Bacopa monniera, a narrative review,” Nutrients, vol. 5, no. 2, pp. 478–497, 2013.
- L. M. Reid and A. M. J. MacLullich, “Subjective memory complaints and cognitive impairment in older people,” Dementia and Geriatric Cognitive Disorders, vol. 22, no. 5-6, pp. 471–485, 2006.
- F. I. Craik, “Memory changes in normal aging,” Current Directions in Psychological Science, vol. 3, no. 5, pp. 155–158, 1994.
- D. F. Hultsch, S. W. S. MacDonald, and R. A. Dixon, “Variability in reaction time performance of younger and older adults,” Journals of Gerontology, Series B: Psychological Sciences and Social Sciences, vol. 57, no. 2, pp. P101–P115, 2002.
- P. Rabbitt and C. Lowe, “Patterns of cognitive ageing,” Psychological Research, vol. 63, no. 3-4, pp. 308–316, 2000.
- T. A. Salthouse, “The processing–speed theory of adult age differences in cognition,” Psychological Review, vol. 103, no. 3, pp. 403–428, 1996.
- P. Verhaeghen and J. Cerella, “Aging, executive control, and attention: a review of meta-analyses,” Neuroscience and Biobehavioral Reviews, vol. 26, no. 7, pp. 849–857, 2002.
- E. M. Zelinski and K. P. Burnight, “Sixteen-year longitudinal and time lag changes in memory and cognition in older adults,” Psychology and Aging, vol. 12, no. 3, pp. 503–513, 1997.
- R. S. Wilson, L. A. Beckett, L. L. Barnes et al., “Individual differences in rates of change in cognitive abilities of older persons,” Psychology and Aging, vol. 17, no. 2, pp. 179–193, 2002.
- J. A. Hardy and G. A. Higgins, “Alzheimer's disease: the amyloid cascade hypothesis,” Science, vol. 256, no. 5054, pp. 184–185, 1992.
- H. Braak and E. Braak, “Frequency of stages of Alzheimer-related lesions in different age categories,” Neurobiology of Aging, vol. 18, no. 4, pp. 351–357, 1997.
- J. C. L. Looi and P. Sachdev, “Structural neuroimaging of the ageing brain,” in The Ageing Brain, P. Sachdev, Ed., pp. 49–62, Swets & Zeitlinger B. V., Sliedrecht, The Netherlands, 2003.
- A. M. J. MacLullich, K. J. Ferguson, I. J. Deary, J. R. Seckl, J. M. Starr, and J. M. Wardlaw, “Intracranial capacity and brain volumes are associated with cognition in healthy elderly men,” Neurology, vol. 59, no. 2, pp. 169–174, 2002.
- V. S. Mattay, F. Fera, A. Tessitore et al., “Neurophysiological correlates of age-related changes in working memory capacity,” Neuroscience Letters, vol. 392, no. 1-2, pp. 32–37, 2006.
- R. Cabeza, “Hemispheric asymmetry reduction in older adults: the HAROLD model,” Psychology and Aging, vol. 17, no. 1, pp. 85–100, 2002.
- P. A. Reuter-Lorenz and K. A. Cappell, “Neurocognitive aging and the compensation hypothesis,” Current Directions in Psychological Science, vol. 17, no. 3, pp. 177–182, 2008.
- D. C. Park and P. Reuter-Lorenz, “The adaptive brain: aging and neurocognitive scaffolding,” Annual Review of Psychology, vol. 60, no. 1, pp. 173–196, 2009.
- P. A. Reuter-Lorenz and D. C. Park, “How does it STAC up? Revisiting the scaffolding theory of aging and cognition,” Neuropsychology Review, vol. 24, no. 3, pp. 355–370, 2014.
- G. B. Chavhan, MRI Made Easy (For Beginners), Japee Brothers Medical Publishers, New Delhi, India, 2nd edition, 2013.
- K. Kantarci, C. R. Jack Jr., Y. C. Xu et al., “Regional metabolic patterns in mild cognitive impairment and Alzheimer's disease: a 1H MRS study,” Neurology, vol. 55, no. 2, pp. 210–217, 2000.
- P. K. Mandal, M. Tripathi, and S. Sugunan, “Brain oxidative stress: detection and mapping of anti-oxidant marker ‘Glutathione’ in different brain regions of healthy male/female, MCI and Alzheimer patients using non-invasive magnetic resonance spectroscopy,” Biochemical and Biophysical Research Communications, vol. 417, no. 1, pp. 43–48, 2012.
- D. P. Auer, B. Pütz, E. Kraft, B. Lipinski, J. Schill, and F. Holsboer, “Reduced glutamate in the anterior cingulate cortex in depression: an in vivo proton magnetic resonance spectroscopy study,” Biological Psychiatry, vol. 47, no. 4, pp. 305–313, 2000.
- A. Bertolino, J. H. Callicott, I. Elman et al., “Regionally specific neuronal pathology in untreated patients with schizophrenia: a proton magnetic resonance spectroscopic imaging study,” Biological Psychiatry, vol. 43, no. 9, pp. 641–648, 1998.
- C. E. Mountford, P. Stanwell, A. Lin, S. Ramadan, and B. Ross, “Neurospectroscopy: the past, present and future,” Chemical Reviews, vol. 110, no. 5, pp. 3060–3086, 2010.
- C. D. Rae, “A guide to the metabolic pathways and function of metabolites observed in human brain 1H magnetic resonance spectra,” Neurochemical Research, vol. 39, no. 1, pp. 1–36, 2014.
- K. K. Haga, Y. P. Khor, A. Farrall, and J. M. Wardlaw, “A systematic review of brain metabolite changes, measured with 1H magnetic resonance spectroscopy, in healthy aging,” Neurobiology of Aging, vol. 30, no. 3, pp. 353–363, 2009.
- R. E. Jung, R. A. Yeo, S. J. Chiulli et al., “Biochemical markers of cognition: a proton MR spectroscopy study of normal human brain,” NeuroReport, vol. 10, no. 16, pp. 3327–3331, 1999.
- A. J. Ross, P. S. Sachdev, W. Wen, M. J. Valenzuela, and H. Brodaty, “Cognitive correlates of 1H MRS measures in the healthy elderly brain,” Brain Research Bulletin, vol. 66, no. 1, pp. 9–16, 2005.
- K. J. Ferguson, A. M. J. MacLullich, I. Marshall et al., “Magnetic resonance spectroscopy and cognitive function in healthy elderly men,” Brain, vol. 125, no. 12, pp. 2743–2749, 2002.
- A. Pfefferbaum, E. Adalsteinsson, D. Spielman, E. V. Sullivan, and K. O. Lim, “In vivo spectroscopic quantification of the N-acetyl moiety, creatine, and choline from large volumes of brain gray and white matter: effects of normal aging,” Magnetic Resonance in Medicine, vol. 41, no. 2, pp. 276–284, 1999.
- D. E. Saunders, F. A. Howe, A. van den Boogaart, J. R. Griffiths, and M. M. Brown, “Aging of the adult human brain: in vivo quantitation of metabolite content with proton magnetic resonance spectroscopy,” Journal of Magnetic Resonance Imaging, vol. 9, no. 5, pp. 711–716, 1999.
- N. Schuff, F. Ezekiel, A. C. Gamst et al., “Region and tissue differences of metabolites in normally aged brain using multislice 1H magnetic resonance spectroscopic imaging,” Magnetic Resonance in Medicine, vol. 45, no. 5, pp. 899–907, 2001.
- K. Kantarci, R. C. Petersen, B. F. Boeve et al., “1H MR spectroscopy in common dementias,” Neurology, vol. 63, no. 8, pp. 1393–1398, 2004.
- K. R. R. Krishnan, H. C. Charles, P. M. Doraiswamy et al., “Randomized, placebo-controlled trial of the effects of donepezil on neuronal markers and hippocampal volumes in Alzheimer's disease,” The American Journal of Psychiatry, vol. 160, no. 11, pp. 2003–2011, 2003.
- R. S. Sohal and W. C. Orr, “The redox stress hypothesis of aging,” Free Radical Biology and Medicine, vol. 52, no. 3, pp. 539–555, 2012.
- N. Parletta, C. M. Milte, and B. J. Meyer, “Nutritional modulation of cognitive function and mental health,” Journal of Nutritional Biochemistry, vol. 24, no. 5, pp. 725–743, 2013.
- R. A. Floyd and K. Hensley, “Oxidative stress in brain aging: implications for therapeutics of neurodegenerative diseases,” Neurobiology of Aging, vol. 23, no. 5, pp. 795–807, 2002.
- S. Papa and V. P. Skulachev, “Reactive oxygen species, mitochondria, apoptosis and aging,” Molecular and Cellular Biochemistry, vol. 174, no. 1-2, pp. 305–319, 1997.
- M. Valko, D. Leibfritz, J. Moncol, M. T. D. Cronin, M. Mazur, and J. Telser, “Free radicals and antioxidants in normal physiological functions and human disease,” The International Journal of Biochemistry and Cell Biology, vol. 39, no. 1, pp. 44–84, 2007.
- F. L. Muller, M. S. Lustgarten, Y. Jang, A. Richardson, and H. Van Remmen, “Trends in oxidative aging theories,” Free Radical Biology and Medicine, vol. 43, no. 4, pp. 477–503, 2007.
- E. E. Essick and F. Sam, “Oxidative stress and autophagy in cardiac disease, neurological disorders, aging and cancer,” Oxidative Medicine and Cellular Longevity, vol. 3, no. 3, pp. 168–177, 2010.
- L. Packer, “Free radical scavengers and antioxidants in prophylaxy and treatment of brain diseases,” in Free Radicals in the Brain, pp. 1–20, Springer, Berlin, Germany, 1992.
- T. Finkel and N. J. Holbrook, “Oxidants, oxidative stress and the biology of ageing,” Nature, vol. 408, no. 6809, pp. 239–247, 2000.
- J. B. Schulz, J. Lindenau, J. Seyfried, and J. Dichgans, “Glutathione, oxidative stress and neurodegeneration,” European Journal of Biochemistry, vol. 267, no. 16, pp. 4904–4911, 2000.
- Y. Artur, B. Herbeth, L. Guémouri, E. Lecomte, C. Jeandel, and G. Siest, “Age-related variations of enzymatic defenses against free radicals and peroxides,” Experientia Supplementum, vol. 62, pp. 359–367, 1992.
- E. Cadenas and K. J. A. Davies, “Mitochondrial free radical generation, oxidative stress, and aging,” Free Radical Biology and Medicine, vol. 29, no. 3-4, pp. 222–230, 2000.
- M. Tosato, V. Zamboni, A. Ferrini, and M. Cesari, “The aging process and potential interventions to extend life expectancy,” Clinical interventions in aging, vol. 2, no. 3, pp. 401–412, 2007.
- H. Sies, “Oxidative stress: oxidants and antioxidants,” Experimental Physiology, vol. 82, no. 2, pp. 291–295, 1997.
- P. Verma, P. Singh, and B. S. Gandhi, “Prophylactic efficacy of Bacopa monnieri on decabromodiphenyl ether (PBDE-209)-induced alterations in oxidative status and spatial memory in mice,” Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 3, pp. 242–247, 2013.
- J. K. Anderson, “Oxidative stress in neurodegeneration: cause or consequence?” Nature Reviews Neuroscience, vol. 5, pp. S18–S25, 2004.
- J. M. Bourre, “Effects of nutrients (in food) on the structure and function of the nervous system: update on dietary requirements for brain. Part 1: micronutrients,” The Journal of Nutrition, Health and Aging, vol. 10, no. 5, pp. 377–385, 2006.
- A. Scalbert and M. Saltmarsh, “Polyphenols: antioxidants and beyond,” The American Journal of Clinical Nutrition, vol. 81, no. 1, supplement, pp. 215S–217S, 2005.
- R. J. Nijveldt, E. van Nood, D. E. C. van Hoorn, P. G. Boelens, K. van Norren, and P. A. M. van Leeuwen, “Flavonoids: a review of probable mechanisms of action and potential applications,” The American Journal of Clinical Nutrition, vol. 74, no. 4, pp. 418–425, 2001.
- F. Rosenfeldt, M. Wilson, G. Lee et al., “Oxidative stress in surgery in an ageing population: pathophysiology and therapy,” Experimental Gerontology, vol. 48, no. 1, pp. 45–54, 2013.
- A. Meister and M. E. Anderson, “Glutathione,” Annual Review of Biochemistry, vol. 52, pp. 711–760, 1983.
- R. Dringen, “Metabolism and functions of glutathione in brain,” Progress in Neurobiology, vol. 62, no. 6, pp. 649–671, 2000.
- M. L. Circu and T. Y. Aw, “Glutathione and modulation of cell apoptosis,” Biochimica et Biophysica Acta—Molecular Cell Research, vol. 1823, no. 10, pp. 1767–1777, 2012.
- P. Maher, “The effects of stress and aging on glutathione metabolism,” Ageing Research Reviews, vol. 4, no. 2, pp. 288–314, 2005.
- M. Raffa, S. Barhoumi, F. Atig, C. Fendri, A. Kerkeni, and A. Mechri, “Reduced antioxidant defense systems in schizophrenia and bipolar I disorder,” Progress in Neuro-Psychopharmacology and Biological Psychiatry, vol. 39, no. 2, pp. 371–375, 2012.
- J. Sian, D. T. Dexter, A. J. Lees et al., “Alterations in glutathione levels in Parkinson's disease and other neurodegenerative disorders affecting basal ganglia,” Annals of Neurology, vol. 36, no. 3, pp. 348–355, 1994.
- G. E. Berger, S. J. Wood, R. M. Wellard et al., “Ethyl-eicosapentaenoic acid in first-episode psychosis. A 1H-MRS study,” Neuropsychopharmacology, vol. 33, no. 10, pp. 2467–2473, 2008.
- P. Gupta, S. Khatoon, P. K. Tandon, and V. Rai, “Effect of cadmium on growth, Bacoside A, and Bacopaside I of Bacopa monnieri (L.), a memory enhancing herb,” The Scientific World Journal, vol. 2014, Article ID 824586, 6 pages, 2014.
- H. K. Singh and B. N. Dhawan, “Neuropsychopharmacological effects of the ayurvedic nootropic Bacopa monniera Linn. (Brahmi),” Indian Journal of Pharmacology, vol. 29, no. 5, pp. S359–S365, 1997.
- D. K. Chowdhuri, D. Parmar, P. Kakkar, R. Shukla, P. K. Seth, and R. C. Srimal, “Antistress effects of bacosides of Bacopa monnieri: modulation of Hsp70 expression, superoxide dismutase and cytochrome P450 activity in rat brain,” Phytotherapy Research, vol. 16, no. 7, pp. 639–645, 2002.
- N. Chatterji, R. P. Rastogi, and M. L. Dhar, “Chemical examination of Bacopa monniera Wettst: part II—the constitution of bacoside A,” Indian Journal of Chemistry, vol. 3, pp. 24–29, 1965.
- S. B. Mahato, S. Garai, and A. K. Chakravarty, “Bacopasaponins E and F: two jujubogenin bisdesmosides from Bacopa monniera,” Phytochemistry, vol. 53, no. 6, pp. 711–714, 2000.
- A. Pengelly, The Constituents of Medicinal Plants. An Introduction to the Chemistry and Therapeutics of Herbal Medicine, Allen & Unwin, Crows Nest, Australia, 2nd edition, 2004.
- S. Ramasamy, L. V. Kiew, and L. Y. Chung, “Inhibition of human cytochrome P450 enzymes by Bacopa monnieri standardized extract and constituents,” Molecules, vol. 19, no. 2, pp. 2588–2601, 2014.
- K. Abascal and E. Yarnell, “Bacopa for the brain: a smart addition to Western medicine,” Alternative and Complementary Therapies, vol. 17, no. 1, pp. 21–25, 2011.
- K. Kishore and M. Singh, “Effect of bacosides, alcoholic extract of Bacopa monniera Linn. (brahmi), on experimental amnesia in mice,” Indian Journal of Experimental Biology, vol. 43, no. 7, pp. 640–645, 2005.
- S. K. Bhattacharya and S. Ghosal, “Anxiolytic activity of a standardized extract of Bacopa monniera: an experimental study,” Phytomedicine, vol. 5, no. 2, pp. 77–82, 1998.
- K. Sairam, M. Dorababu, R. K. Goel, and S. K. Bhattacharya, “Antidepressant activity of standardized extract of Bacopa monniera in experimental models of depression in rats,” Phytomedicine, vol. 9, no. 3, pp. 207–211, 2002.
- C. Calabrese, W. L. Gregory, M. Leo, D. Kraemer, K. Bone, and B. Oken, “Effects of a standardized Bacopa monnieri extract on cognitive performance, anxiety, and depression in the elderly: a randomized, double-blind, placebo-controlled trial,” Journal of Alternative and Complementary Medicine, vol. 14, no. 6, pp. 707–713, 2008.
- S. Roodenrys, D. Booth, S. Bulzomi, A. Phipps, C. Micallef, and J. Smoker, “Chronic effects of Brahmi (Bacopa monnieri) on human memory,” Neuropsychopharmacology, vol. 27, no. 2, pp. 279–281, 2002.
- S. Aguiar and T. Borowski, “Neuropharmacological review of the nootropic herb Bacopa monnieri,” Rejuvenation Research, vol. 16, no. 4, pp. 313–326, 2013.
- Y. B. Tripathi, S. Chaurasia, E. Tripathi, A. Upadhyay, and G. P. Dubey, “Bacopa monniera Linn. as an antioxidant: mechanism of action,” Indian Journal of Experimental Biology, vol. 34, no. 6, pp. 523–526, 1996.
- A. Russo, A. A. Izzo, F. Borrelli, M. Renis, and A. Vanella, “Free radical scavenging capacity and protective effect of Bacopa monniera L. on DNA damage,” Phytotherapy Research, vol. 17, no. 8, pp. 870–875, 2003.
- A. Dar and S. Channa, “Calcium antagonistic activity of Bacopa monniera on vascular and intestinal smooth muscles of rabbit and guinea-pig,” Journal of Ethnopharmacology, vol. 66, no. 2, pp. 167–174, 1999.
- D. Rai, G. Bhatia, G. Palit, R. Pal, S. Singh, and H. K. Singh, “Adaptogenic effect of Bacopa monniera (Brahmi),” Pharmacology Biochemistry and Behavior, vol. 75, no. 4, pp. 823–830, 2003.
- S. Channa, A. Dar, S. Anjum, and M. Yaqoob, “Anti-inflammatory activity of Bacopa monniera in rodents,” Journal of Ethnopharmacology, vol. 104, no. 1-2, pp. 286–289, 2006.
- M. Dhanasekaran, B. Tharakan, L. A. Holcomb, A. R. Hitt, K. A. Young, and B. V. Manyam, “Neuroprotective mechanisms of ayurvedic antidementia botanical Bacopa monniera,” Phytotherapy Research, vol. 21, no. 10, pp. 965–969, 2007.
- N. Uabundit, J. Wattanathorn, S. Mucimapura, and K. Ingkaninan, “Cognitive enhancement and neuroprotective effects of Bacopa monnieri in Alzheimer's disease model,” Journal of Ethnopharmacology, vol. 127, no. 1, pp. 26–31, 2010.
- J. Mathew, G. Gangadharan, K. P. Kuruvilla, and C. S. Paulose, “Behavioral deficit and decreased GABA receptor functional regulation in the hippocampus of epileptic rats: effect of Bacopa monnieri,” Neurochemical Research, vol. 36, no. 1, pp. 7–16, 2011.
- M. K. Saraf, S. Prabhakar, and A. Anand, “Bacopa monniera alleviates Nω-nitro-l-arginine-induced but not MK-801-induced amnesia: a mouse Morris water maze study,” Neuroscience, vol. 160, no. 1, pp. 149–155, 2009.
- R. R. Kumar, K. Kathiravan, and R. Muthusamy, “Bacopa monniera a potent neuroprotector against transient global cerebral ischemia induced hippocampal damage and memory function,” International Journal of Anatomoical Sciences, vol. 3, no. 2, pp. 26–32, 2012.
- G. K. Shinomol and M. M. S. Bharath, “Neuromodulatory propensity of Bacopa monnieri leaf extract against 3-nitropropionic acid-induced oxidative stress: in vitro and in vivo evidences,” Neurotoxicity Research, vol. 22, no. 2, pp. 102–114, 2012.
- K. J. Gohil and J. J. Patel, “A review on Bacopa monniera: current research and future prospects,” International Journal of Green Pharmacy, vol. 4, no. 1, pp. 1–9, 2010.
- D. Sudharani, K. L. Krishna, K. Deval, A. K. Safia, and Priya, “Pharmacological profiles of Bacopa monnieri: a review,” Inernational Journal of Pharmacy, vol. 1, no. 1, pp. 15–23, 2011.
- N. Limpeanchob, S. Jaipan, S. Rattanakaruna, W. Phrompittayarat, and K. Ingkaninan, “Neuroprotective effect of Bacopa monnieri on beta-amyloid-induced cell death in primary cortical culture,” Journal of Ethnopharmacology, vol. 120, no. 1, pp. 112–117, 2008.
- G. K. Shinomol, “Bacopa monnieri modulates endogenous cytoplasmic and mitochondrial oxidative markers in prepubertal mice brain,” Phytomedicine, vol. 18, no. 4, pp. 317–326, 2011.
- G. K. Shinomol and M. M. S. Bharath, “Neuromodulatory propensity of bacopa monnieri leaf extract against 3-nitropropionic acid-induced oxidative stress: in vitro and in vivo evidences,” Neurotoxicity Research, vol. 22, no. 2, pp. 102–114, 2012.
- K. Anbarasi, G. Vani, K. Balakrishna, and C. S. S. Devi, “Effect of bacoside A on brain antioxidant status in cigarette smoke exposed rats,” Life Sciences, vol. 78, no. 12, pp. 1378–1384, 2006.
- A. Jyoti and D. Sharma, “Neuroprotective role of Bacopa monniera extract against aluminium-induced oxidative stress in the hippocampus of rat brain,” NeuroToxicology, vol. 27, no. 4, pp. 451–457, 2006.
- M. K. Saraf, S. Prabhakar, and A. Anand, “Neuroprotective effect of Bacopa monniera on ischemia induced brain injury,” Pharmacology Biochemistry and Behavior, vol. 97, no. 2, pp. 192–197, 2010.
- A. Russo, F. Borrelli, A. Campisi, R. Acquaviva, G. Raciti, and A. Vanella, “Nitric oxide-related toxicity in cultured astrocytes: effect of Bacopa monniera,” Life Sciences, vol. 73, no. 12, pp. 1517–1526, 2003.
- T. Anand, M. Naika, M. S. L. Swamy, and F. Khanum, “Antioxidant and DNA damage preventive properties of Bacopa monniera (L) Wettst,” Free Radicals and Antioxidants, vol. 1, no. 1, pp. 84–90, 2011.