Inhibitory Effects of Chrysanthemum boreale Essential Oil on Biofilm Formation and Virulence Factor Expression of Streptococcus mutans

Table 1

Oligonucleotide primers that were used in this study.

Gen	Gene description	Primer sequences (5′-3′)
gtfB	Glucosyltransferase-I	Forward: Reverse:	AGCAATGCAGCCAATCTACAAAT; ACGAACTTTGCCGTTATTGTCA
gtfC	Glucosyltransferase-SI	Forward: Reverse:	GGTTTAACGTCAAAATTAGCTGTATTAGC; CTCAACCAACCGCCACTGTT
gtfD	Glucosyltransferase-S	Forward: Reverse:	ACAGCAGACAGCAGCCAAGA; ACTGGGTTTGCTGCGTTTG
brpA	Biofilm regulatory protein A	Forward: Reverse:	GGAGGAGCTGCATCAGGATTC; AACTCCAGCACATCCAGCAAG
spaP	Cell surface antigen SpaP	Forward: Reverse:	GACTTTGGTAATGGTTATGCATCAA; TTTGTATCAGCCGGATCAAGTG
gbpB	Secreted antigen GbpB/SagA	Forward: Reverse:	ATGGCGGTTATGGACACGTT; TTTGGCCACCTTGAACACCT
relA	GTP pyrophosphokinase	Forward: Reverse:	ACAAAAAGGGTATCGTCCGTACAT; AATCACGCTTGGTATTGCTAATTG
vicR	Response regulator	Forward: Reverse:	TGACACGATTACAGCCTTTGATG; CGTCTAGTTCTGGTAACATTAAGTCCAATA
16S rRNA	16S rRNA	Forward: Reverse:	CCTACGGGAGGCAGCAGTAG; CAACAGAGCTTTACGATCCGAAA

Based on the NCBI S. mutans genome database.