Shikonin Induces Apoptosis, Necrosis, and Premature Senescence of Human A549 Lung Cancer Cells through Upregulation of p53 Expression

Figure 2

Effects of shikonin on apoptosis and necrosis of A549 cells. (a) Representative flow cytometry results. A549 cells were treated with shikonin (SHK) at different concentrations, stained with Annexin V-FITC/PI and then subjected to flow cytometry. Viable cells (scattered in left lower quadrant of the graphs) decreased whereas early-apoptotic (right lower quadrant), late-apoptotic (right upper quadrant), and necrotic (left upper quadrant) cells increased to various extents in response to SHK treatment. (b) Quantitative results from flow cytometry. Data were represented as mean ± SEM from at least three independent experiments. , and v.s. Cont.

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