A Novel Korean Red Ginseng Compound Gintonin Inhibited Inflammation by MAPK and NF-κB Pathways and Recovered the Levels of mir-34a and mir-93 in RAW 264.7 Cells

Figure 3

Suppressive effect of gintonin on protein expression of iNOS and COX-2. For protein expressions, RAW 264.7 cells were seeded in 6-well plate and treated with indicated concentrations of gintonin (6.25 μg/mL–50 μg/mL) for 30 min and then stimulated with LPS (0.1 μg/mL) for 18 h. Total protein was extracted from RAW 264.7 cells using Pro-prep lysis reagent. Protein quantitation was done by Pro-Measure and then proteins were run on SDS-PAGE and analyzed by ECL chemiluminescence. Western blot images are representative of 3 independent experiments. Bar values of compared to LPS were considered statistically significant.