A Novel Korean Red Ginseng Compound Gintonin Inhibited Inflammation by MAPK and NF-κB Pathways and Recovered the Levels of mir-34a and mir-93 in RAW 264.7 Cells
MAPK pathway inhibition by gintonin. RAW 264.7 cells seeded in 6-well plate were pretreated with gintonin (6.25 μg/mL–50 μg/mL) and then stimulated with or without LPS (0.1 μg/mL) for 18 h. Total proteins were extracted and run on SDS-PAGE. Afterwards membranes were incubated with primary antibodies overnight followed by treatment with secondary antibody for 2 h. Proteins were then analyzed by ECL chemiluminescence system. Images are representative of at least 3 independent experiments. Data are mean ± SD (); and compared with LPS only. (a) Phosphorylation of P-MKK compared to T-MKK. (b) Phosphorylation of P-MEK compared to T-MEK. (c) Phosphorylation of P-JNK compared to T-JNK. (d) Phosphorylation of P-p38 compared to T-p38. (e) Phosphorylation of P-ERK compared to T-ERK.