A Novel Korean Red Ginseng Compound Gintonin Inhibited Inflammation by MAPK and NF-κB Pathways and Recovered the Levels of mir-34a and mir-93 in RAW 264.7 Cells

Figure 7

Recovery of mir-34a and mir-93 by gintonin. (a) RAW 264.7 cells were treated with LPS (0.1–5 μg/mL) for 18 h. (b) RAW 264.7 cells were treated with gintonin (12.5 μg/mL–50 μg/mL) in indicated concentrations and then stimulated with or without LPS (5 μg/mL) for 18 h. Total RNA was extracted and mRNA expression of mir-34a was observed using real-time PCR. Bar values are ± SEM of at least 3 independent experiments. and compared to LPS only. (c) RAW 264.7 cells were treated with gintonin (12.5 μg/mL–50 μg/mL) in indicated concentrations and then stimulated with or without LPS (5 μg/mL) for 18 h. Total RNA was extracted and then mRNA expression of mir-93 was observed using real-time PCR. Bar values are ± SEM of at least 3 independent experiments. Statistic comparison is same as for mir-34a.

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