Neurosupportive Role of Vanillin, a Natural Phenolic Compound, on Rotenone Induced Neurotoxicity in SH-SY5Y Neuroblastoma Cells

Figure 2

Effect of vanillin on rotenone induced cytotoxicity (MTT assay) in SH-SY5Y neuroblastoma cells. Cell viability was determined by measuring MTT method. (a) shows the dose-dependent effect of rotenone (5, 10, 50, 100, and 200 nM) induced cell toxicity after 24 h. An approximately half-maximal inhibition of cell viability was obtained at 100 nM rotenone concentration. (b) shows the dose-dependent effect of vanillin at various concentrations. Low concentrations (5, 10, 20, 50, 100, and 200 nM) did not induce any toxicity after 24 h treatment, whereas slight toxicity was induced at 500 μM concentration. Values are expressed as the percentage of the untreated control and represented as mean ± SD of four independent experiments in each group.

(a)

(b)