Neurosupportive Role of Vanillin, a Natural Phenolic Compound, on Rotenone Induced Neurotoxicity in SH-SY5Y Neuroblastoma Cells

Figure 7

The effect of vanillin on the expressions of apoptotic and signaling markers. Lane 1: control; 2: rotenone; 3: vanillin + rotenone; and 4: vanillin. (a), (b), and (c) show the expressions of Bax; caspase-3, caspase-8, and caspase-9 cyt-c in cytosol were increased while the expressions of Bcl-2 and cyt-c in mitochondria were significantly decreased by the rotenone treated group as compared with control. Pretreatment with vanillin gradually restored the imbalanced expression profile of these proteins. (d) Rotenone treatment stimulates the expressions of p-JNK, p-P38, and p-ERK as compared with control. Pretreatment with vanillin decreases the expressions of p-JNK, p-P38, and p-ERK significantly. Immunoblots are representative of at least four independent experiments. Values are given as mean ± SD in each group. compared to control and compared to rotenone group (DMRT).

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