In Vitro Evaluation of the Effects of Eurycoma longifolia Extract on CYP-Mediated Drug Metabolism

Figure 2

Representative MRM chromatograms of (a) control and (b) Eurycoma longifolia extracts-treated human liver microsome samples. Human liver microsomal fraction was incubated with the substrate mixture, NADPH-generating system, and Eurycoma longifolia extracts (1000 μg/mL) for 30 min and the formation of the CYP-specific metabolites was determined by LC-MS/MS. (A) Acetaminophen; (B) 7-OH-coumarin; (C) 6-OH-paclitaxel; (D) 4-OH-diclofenac; (E) 4-OH-mephenytoin; (F) dextrorphan; (G) 1-OH-madazolam; (H) terfenadine (IS).

(a)

(b)