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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 635975, 7 pages
Research Article

Assessment of Lemon Balm (Melissa officinalis L.) Hydrogels: Quality and Bioactivity in Skin Cells

1Department of Clinical Pharmacy, Lithuanian University of Health Sciences, A. Mickevičiaus Street 9, Kaunas, Lithuania
2Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, A. Mickevičiaus Street 9, Kaunas, Lithuania
3Neuroscience Institute, Lithuanian University of Health Sciences, Eivenių Street 4, Kaunas, Lithuania

Received 22 July 2015; Revised 22 September 2015; Accepted 7 October 2015

Academic Editor: Yuri Clement

Copyright © 2015 Kristina Ramanauskienė et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of the study was to design gels with lemon balm extract, assess their quality, and investigate the effect of rosmarinic acid on skin cells in normal conditions and under oxidative stress. Methods. The quantities of rosmarinic acid (RA) released from gels were evaluated by applying the HPLC technique. HaCaT cell viability was assessed by using the MTT method. ROS generation was measured using DCFH-DA dye. The results showed that the gelling material affected the release of RA content from gels. Lower and slower RA content release was determined in carbomer-based gels. After 6 hours of biopharmaceutical research in vitro, at least 4% of RA was released from the gel. The results of the biological studies on HaCaT cells demonstrated that, in the oxidative stress conditions, RA reduced intracellular ROS amounts to 28%; 0.25–0.5 mg/mL of RA increased cell viability by 10–24% and protected cells from the damage caused by H2O2. Conclusions. According to research results, it is appropriate to use a carbomer as the main gelling material, and its concentration should not exceed 1.0%. RA, depending on the concentration, reduces the amount of intracellular ROS and enhances cell viability in human keratinocytes in oxidative stress conditions.