Integrated Treatment of Aqueous Extract of Solanum nigrum-Potentiated Cisplatin- and Doxorubicin-Induced Cytotoxicity in Human Hepatocellular Carcinoma Cells

Figure 5

Activation of selected protein markers in HCC cells treated with AE-SN and either cisplatin or doxorubicin. (a) Cells were treated with a control medium, namely, 5 μM cisplatin or 2 μM doxorubicin with 0 or 1.0 mg/mL of AE-SN for 48 h. The activation of LC-3 A/B and caspase-7 was determined using western blotting analysis. GAPDH served as an internal control. (b) Semiquantitation of the LC-3 A/B II and cleaved caspase-7 in the Hep3B and HepJ5 cells. The data are presented as the mean ± standard deviation. indicates statistical significance in comparison with the previous group, namely, Con versus AESN (vehicle control versus AE-SN), Cs versus Cs + AESN, and Dx versus Dx + AESN (two-tailed Student’s -test, ).

(a)

(b)