Orostachys japonicus Inhibits Expression of the TLR4, NOD2, iNOS, and COX-2 Genes in LPS-Stimulated Human PMA-Differentiated THP-1 Cells by Inhibiting NF-κB and MAPK Activation

Figure 3

Effects of O. japonicus on LPS-induced iNOS and COX-2 mRNA and protein expression in THP-1 cells. Cells were pretreated for 20 h with various concentrations of O. japonicus (10 or 25 μg/mL) before exposure to LPS (1 μg/mL) for 4 h. Levels of (a) iNOS and (b) COX-2 mRNA were measured using real-time PCR. The crossing points of iNOS and COX-2 with β-actin were entered into the formula , and relative amounts were quantified. iNOS (c) and COX-2 (d) protein levels were determined by immunoblotting. Densitometric analyses are presented as the relative ratios of iNOS and COX-2 with β-actin. The data represent the means ± SD of three independent samples. compared with LPS stimulation alone.

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