Research Article
Alleviation of Carbon-Tetrachloride-Induced Liver Injury and Fibrosis by Betaine Supplementation in Chickens
Table 1
List of primer sequences for quantitative real-time PCR.
| | Gene symbol | Primer sequences 5′-3′ | Anneal temp. (°C) | GenBank accession number | Product size (bp) |
| | IL-6 | F: CTCCTCGCCAATCTGAAGTC | 60 | NM_204628.1 | 164 | | R: GGATTGTGCCCGAACTAAAA |
| | TGF-β1 | F: AGCCACAGCATCTTCTTCGT | 58.4 | JQ423909.1 | 162 | | R: ATTGCCGTAACCCTGGTACA |
| | ACTA2 | F: CACCCAACTCTGCTGACTGA | 61.4 | NM_001031229.1 | 176 | | R: ACACCATCCCCAGAGTCAAG |
| | COL1A1 | F: GAACCCCAAGGAGAAGAAGC | 58.4 | XM_423116.4 | 167 | | R: TCTTGCAGTGGTAGGTGACG |
| | COL3A1 | F: AGGCTGAAGGAAACAGCAAA | 58.4 | NM_205380.2 | 102 | | R: TGCGCGTTCTGTATTCAAAG |
| | DPP4 | F: GGTGGGCACACTTTCCTAAA | 60 | NM_001031255.1 | 101 | | R: TTGAGCATTCAGGCAACAAG |
| | GST | F: ACCTGCTTCAAAAGCTGGAA | 58.4 | NM_205412.1 | 130 | | R: GTTAGCCAGGACTGCCAGAG |
| | DDT | F: TTGCTGTTGCACTTCACTCC | 58.4 | NM_001030667.1 | 152 | | R: GAAGCCAGTGCATGTTCTCA |
| | GLUL | F: GCTCAGTGGGGAAGACTCAG | 58.4 | NM_205493.1 | 177 | | R: GGTCCAGAACGTGGTGAAGT |
| | AACS | F: CCAGCTAATGCGTGCTGATA | 63 | NM_001006184.1 | 162 | | R: TTGACTGCTTGTTGCTGTCC |
| | ACTB | F: GTGATGGACTCTGGTGATGG | 62 | NM_205518.1 | 151 | | R: TGGTGAAGCTGTAGCCTCTC |
|
|
IL-6, interleukin-6; TGF-β1, transforming growth factor-β1; ACTA2, α-smooth muscle actin; COL1A1, collagen type 1-α1; COL3A1, collagen type 3-α1; DPP4, dipeptidyl-peptidase 4; GST, glutathione S-transferase; DDT, d-dopachrome tautomerase; GLUL, glutamine synthetase; AACS, acetoacetyl-CoA synthase; and ACTB, β-actin.
|
