Comparison of Alcian Blue, Trypan Blue, and Toluidine Blue for Visualization of the Primo Vascular System Floating in Lymph Ducts

Figure 5

Images of a primo node extracted from the lymph duct. (a) A primo node stained by Alcian blue was extracted from a lymph vessel. One of the primo vessels connected to the node was cut off during the extraction process, and only one side was kept. The primo vessel was a bundle of two or more subvessels. (b) DAPI images of the same primo node as in (a), which contained many nuclei. Two rod-shaped nuclei (arrows) can be seen in the primo vessel.

(a)

(b)