Research Article

Yokukansan Increases 5-HT1A Receptors in the Prefrontal Cortex and Enhances 5-HT1A Receptor Agonist-Induced Behavioral Responses in Socially Isolated Mice

Figure 3

Yokukansan (YKS) cotreatment did not affect expression of 5-H receptor protein in the PFC of isolation-stressed mice. Tissue homogenates for Western blot analysis were prepared from a cell fraction containing cytoplasm and plasma membranes but not nuclei (see Section 2). (a) Representative images of Western blots. Immunoreactive 5-H receptor (65 kD) and actin (43 kD) were detected as single bands. (b) Results of densitometric analysis. Each column is the mean ± SEM (). There were no significant differences among groups.
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