A Bio-Guided Fractionation to Assess the Inhibitory Activity of Calendula officinalis L. on the NF-κB Driven Transcription in Human Gastric Epithelial Cells

Figure 1

Effect of CH₂Cl₂ extract on the NF-κB driven transcription. AGS cells were transiently transfected with NF-κB-luc plasmid and treated with TNF-α in the presence of dichloromethane extract (1–20 μg/mL) for 24 h. The NF-κB driven transcription was proportional to luciferase signal measured. , , and .