Traditional Herbal Formula Banhasasim-tang Exerts Anti-Inflammatory Effects in RAW 264.7 Macrophages and HaCaT Keratinocytes

Figure 4

Effects of Banhasasim-tang (BHSST) on production of inflammatory mediators and cytokine in RAW 264.7 cells. Cells were pretreated with BHSST (250, 500 or 1000 μg/mL) for 4 h and then stimulated with LPS (1 μg/mL) for 20 h. NO production was measured in the culture supernatant with the Griess reaction (a). The levels of PGE₂ (b) and IL-6 (c) released into the culture medium were assessed using commercially available ELISA kits. L-NMMA and indomethacin (ID) were used as positive controls for NO and PGE₂, respectively. Values are expressed as mean ± SEM of three independent experiments. Each bar represents the mean of three independent experiments. versus vehicle control cells; and versus LPS-treated cells.

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