Traditional Herbal Formula Banhasasim-tang Exerts Anti-Inflammatory Effects in RAW 264.7 Macrophages and HaCaT Keratinocytes

Figure 8

Effect of Banhasasim-tang (BHSST) on TNF-α and IFN-γ-induced STAT1 activation in HaCaT cells. (a) Expression of total and phosphorylated STAT1 was determined by Western blotting. Cells were cotreated with BHSST (125, 250, or 500 μg/mL), and TNF-α and IFN-γ (TI, each 10 ng/mL) for 30 min. Silymarin (12.5 or 25 μg/mL) was used as a positive control. Results are representative of at least three independent experiments. (b) Cellular localization of STAT1 was examined by immunofluorescence staining. Cells were cotreated with BHSST (500 μg/mL), and TI for 30 min. The cells were fixed with 4% (v/v) methanol-free formaldehyde solution (pH 7.4), stained with anti-STAT1 (red). The stained cells were mounted with medium containing DAPI (blue) and visualized under an Olympus FLUOVIEW FV 10i confocal microscope.

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