Herb-Drug Pharmacokinetic Interaction of a Traditional Chinese Medicine Jia-Wei-Xiao-Yao-San with 5-Fluorouracil in the Blood and Brain of Rat Using Microdialysis
Table 4
Pharmacokinetic parameters of 5-FU in blood and the brain microdialysates after 5-FU administration (100 mg/kg, iv) alone and pretreated with a different dose of JWXYS (600, 1200, or 2400 mg/kg/day for 5 consecutive days) ( = 6).
Parameter
5-FU
5-FU + JWXYS (600 mg/kg/day, p.o.)
5-FU + JWXYS (1200 mg/kg/day, p.o.)
5-FU + JWXYS (2400 mg/kg/day, p.o.)
Blood
AUC (min μg/mL)
5020 ± 443
5516 ± 486
4801 ± 244
6159 ± 898
(μg/mL)
107 ± 10
111 ± 9
98 ± 6
107 ± 8
(min)
11.0 ± 0.6
13.1 ± 1.6
13.3 ± 1.6
25.6 ± 5.2
CL (mL/min per kg)
20.8 ± 2.0
18.7 ± 1.4
21.0 ± 1.0
17.6 ± 2.6
Vd (mL/kg)
448 ± 51
406 ± 45
495 ± 76
575 ± 41
Brain
AUC (min μg/mL)
894 ± 141
744 ± 133
834 ± 126
1114 ± 128
(μg/mL)
11.8 ± 1.8
9.3 ± 1.2
10.1 ± 1.6
11.2 ± 1.1
(min)
31.9 ± 3.6
28.9 ± 3.9
33.1 ± 2.9
47.2 ± 7.3
CL (mL/min per kg)
120 ± 23
147 ± 28
122 ± 17.5
82.9 ± 11.0
Vd (mL/kg)
6351 ± 953
7581 ± 1080
7114 ± 827
6525 ± 819
Ratio of penetration (%)
17.8 ± 2.8
14.6 ± 2.6
16.4 ± 2.5
24.2 ± 2.9
Data are expressed as mean ± S.E.M. < 0.05 comparing with that in 5-FU alone group. The ratio of penetration (%) was calculated as follows: (AUCbrain/AUCblood) 100. AUCbrain meant AUC of brain in each group and AUCblood meant AUC of blood in 5-FU alone group.
